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IBC induced caspase-3 dependent apoptosis in MDA-MB-231 cells. (A) Western blotting analysis of caspase-3. (B) Cell viability following treatment with IBC (40 µM) for 24 h alone or with the pan-caspase inhibitor z-VAD-FMK (20 µM) pre-treatment as measured by the MTT assay. (C) Annexin V-FITC/PI analysis following treatment with IBC with or without pre-treatment with z-VAD-FMK. * p < 0.05 compared between the groups of IBC and IBC with z-VAD-FMK.
