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. 2022 Aug 29;36(11):2678–2689. doi: 10.1038/s41375-022-01686-y

Fig. 6. Inhibition of glycolysis does not restore defective adipogenesis in tMN BMSC.

Fig. 6

A Schematic showing that Healthy BMSC were cultured in adipogenesis-induction media with or without OXPHOS inhibitor (IACS-010759) for 14 days followed by 7 days without the inhibitor and adipogenesis was assessed after total of 21 days culture. The number of adipocytes was enumerated by counting Nile Red-labelled cells and DAPI-labelled cell nuclei. The bar represents average of results from three Healthy BMSC; B Healthy (n = 3) and tMN BMSC (n = 3) were cultured under adipogenic induction conditions for 4 weeks either with or without 1 mM and 2 mM of 2DG, a glycolysis inhibitor, and number of adipocytes were evaluated by counting Nile Red-labelled cells and DAPI-labelled cell nuclei. All bars indicate mean, and all error bars indicate SD. Scale bars indicate 100 µm. Mann–Whitney test was used to detect statistically significant differences between cohorts. Asterisks display P values **P < 0.05.