Fig. 4.

Senolytic treatment effectively cleared senescent cells in old mice following mechanical overload (MOV). (a) Study design schematic for the synergist ablation-induced MOV of the plantaris muscle of adult and old mice. (b) Representative image for senescence-associated beta-galactosidase (SA β-Gal) staining. (c) Quantification of SA β-Gal + cells per 10⁵ μm² in old vehicle sham (open blue bar), old D + Q sham (open red bar), old vehicle 14-day MOV (hashed blue bar), and old D + Q 14-day MOV (hashed red bar). N = 7–9/group. (d) Representative image of p21 immunohistochemistry in the plantaris 14 days following MOV in a vehicle-treated old mouse. (f) Quantification of p21 + cells per 10⁵ μm² in old vehicle sham (open blue bar), old D + Q sham (open red bar), old vehicle 14-day MOV (hashed blue bar), and old D + Q 14-day MOV (hashed red bar). N = 7–9/group. *Significance versus sham controls for a given treatment group. ^Significance versus vehicle-treated mice for a given time point