Figure 8.

Increased hepatic mitophagosome processing into lysosomes in vivo after acute ethanol. GFP-LC3 Tg mice were injected with RhDex and gavaged with saline (A) or ethanol (4 g/kg, B) ~18 h later, as described in Materials and Methods. Intravital multiphoton microscopy of GFP-LC3 (left panels) and RhDex (middle panels) was performed using a 60x silicone oil objective lens at ~4 h after ethanol treatment. Right panels are overlays of the RhDex and GFP-LC3 images. Scale bar: 2 µm. Shown are representative images from 3 mice/group. (C), quantification of RhDex-positive areas by image analysis using ImageJ. Colocalization of RhDex and GFP-LC3 puncta was analyzed using ImageJ by 2 different methods and expressed as Spearman’s rank correlation value (D) and Pearson’s R value (E), respectively. Values are means ± SEM (n = 3/group). *, p < 0.05, **, p < 0.01 vs no ethanol.