Figure 9.

Interaction of ROCK1 with BECN1 in vessels of the ipsilateral thalamus after MCAO. (A) Double-staining shows ROCK1 (green) to be colocalized with BECN1⁺ cells (red) in vessels of the ipsilateral thalamus (arrows). Scale bar: 50 μm. (B) Co-immunoprecipitation was performed with vessels extracts of the ipsilateral thalamus in the sham- and MCAO-operated animals by using ROCK1-crosslinked agarose, and the resulting immunoprecipitation was blotted with the indicated antibodies. The relative levels of ROCK1 and BECN1 of input were detected by immunoblotting. n = 3. (C-E) Immunoblotting shows the levels of p-BECN1 (Thr119) and total BECN1 in the ipsilateral thalamic vessels of the sham-operated, MCAO, Scramble-siRNA, Rtn4-siRNA, IgG-Fc, and RTN4-Δ20-Fc groups at 7 days after MCAO, respectively. (F-H) Quantitative analysis of p-BECN1 expression relative to GAPDH in the ipsilateral thalamic vessels of different groups. n = 4, data are expressed as median ± interquartile range. *P < 0.05, compared with the respective control group.