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. 2022 Mar 14;18(11):2697–2710. doi: 10.1080/15548627.2022.2047343

Figure 5.

Figure 5.

Trehalose stimulates cholesterol efflux and reduces lipid load and inflammation in mye-macrophages. Bone marrow-derived macrophages (BMDMs) were treated with myelin for a short (Mye-24) or prolonged (Mye-72) time and were additionally stimulated with trehalose (TOH) for the final 6 h of myelin-ingestion. (A) Representative images of Oil Red O staining. Scale bar: 50 µm. (n = 4 wells). (B) Capacity of cells to efflux cholesterol and normalized to non-stimulated cells (n = 3). (C) Relative nitric oxide (NO) production by BMDMs stimulated with LPS for 18 h after TOH and myelin treatment (n = 6). (D-E) mRNA expression of pro-inflammatory genes in BMDMs stimulated with LPS for 6 h after TOH and myelin treatment (n = 6). All data are represented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001.