Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Oct 25;25(11):105439. doi: 10.1016/j.isci.2022.105439

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

ATP binding and hydrolysis of human DMC1 mutants

(A) ATP binding of human DMC1 variants measured as a change of the fluorescence emission intensity of TNP-ATP upon binding to DMC1 in the presence of ATP (1 mM) and 1-mM MgCl₂ (mean ± SD; n = 3).

(B) ATP binding of human DMC1 variants measured as a change of the fluorescence emission intensity of TNP-ATP upon binding to DMC1 in the presence of ATP (1 mM) and 1-mM CaCl₂ (mean ± SD; n = 3).

(C) ATP binding of human DMC1 variants measured as a change of the fluorescence emission intensity of TNP-ATP upon binding to DMC1 in the presence of ATP (1 mM) and absence of bivalent ion (mean ± SD; n = 3).

(D) ATP hydrolysis of DMC1 variants (2 μM) analyzed using the colorimetric phosphate detection assay in the presence of 90-mer ssDNA (6.3-μM nucleotides) and MgCl₂ (1 mM) (mean ± SD; n = 3).