The authors report that there were errors in the primer sequences in Supplemental Tables S2 and S3 and provide the revised tables shown below. The authors state that the corrections do not affect the results and conclusions of the article and apologize for any confusion this may have caused.
Table S2: sgRNAs used in this study.
| CRISPR-Cas9 editing | Sequences (5′-3′) | |
|---|---|---|
| CBE+47-KO | sgRNA-L | CAACAGTTGCTGTACTTGCA |
| sgRNA-R | CCACATGATGGTGATGTCGA | |
| ΔE1 | sgRNA1L | TCTCTCTCATTGTTATCTGT |
| sgRNA1R | ACCTATGGACAAACTATTCA | |
| ΔE2 | sgRNA2L | ATATAGCGAGAAAACTACGA |
| sgRNA2R | CTAAACTCACTTCAAGCGGC | |
| ΔE3 | sgRNA3L | GGCCTCCCCTTGAGAAATTC |
| sgRNA3R | TTGGAGGTCACCACAACGTG |
Table S3. Primers were used to identify the deletion clones in this study.
| Primer names | Sequences (5′-3′) | ||
|---|---|---|---|
| CBE+47-KO | Primer | forward | GCCTGACAAGGTCACAAGACT |
| reverse | CGGACAGTGAGCTTCCTCAAA | ||
| ΔE1 | Primer 1 | forward | GCTCCTTGCTTGCCTTCAAAA |
| reverse | AGCTGATTGTCTTCCCACCC | ||
| ΔE2 | Primer 2 | forward | CGCGCTTTCTATGGCAGAAT |
| reverse | AAACTTCCAGACTCGGTGGT | ||
| ΔE3 | Primer 3 | forward | CCCTCCAGCTTTCTTTAGACCA |
| reverse | AGCAGACACTGCCCATTTGA | ||
VOLUME 296 (2021) 100413
