The legend for Fig. 8C was missing in the article as originally published. The corrected Fig. 8 legend is below. The legend has been corrected in the online version of the article, which now differs from the print version as originally published.
FIGURE 8. Acidity reduces innate responses in vivo. Mice were injected i.p. with 1 ml of 500 mM MOPS (pH 6.0) or HEPES (pH 7.2) with or without 10 μg of LPS.(A) Serum was collected 2 h after injection for TNF-α ELISA and 6 h later for IL-6 and CCL-2 ELISAs. Data were combined from two experiments, each with n = 4–6 per group. (B) Mouse peritoneal cells were collected 6 h after injection and macrophages were allowed to adhere in cRPMI for 3 h. The macrophages were then incubated in fresh cRPMI medium for 12 h before the culture supernatant was tested for cytokine secretion. Data were combined from two experiments, each with n = 3 or 4. *p < 0.05, **p < 0.01, ***p < 0.001. (C) Six h after i.p. LPS injection, peritoneal cells were counted and the numbers of neutrophils (CD11b+, Ly6G+) and macrophages (CD11b+, F4/80+) were calculated after flow cytometric analysis. The cell number changes after LPS i.p. were also plotted. Data were combined from 2 experiments, each with n = 3 or 4.