REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Monoclonal ANTI-FLAG M2 antibody	Sigma-Aldrich	Cat# F3165; RRID:AB_259529
ANTI-FLAG M2 Affinity Gel	Sigma-Aldrich	Cat# A2220; RRID:AB_10063035
Myc-Tag Mouse mAb	Cell Signaling Technology	Cat# 2276; RRID:AB_331783
Anti-Histone H3 antibody	Huabio	Cat# M1306-4
Beta Actin Monoclonal antibody	Proteintech	Cat# 66009-1-Ig; RRID:AB_2687938
Anti-Benzoyllysine Rabbit mAb	PTM Biolabs	Cat# PTM-761
Anti- Benzoyllysine antibody conjugated agarose beads	PTM Biolabs	Cat# PTM-763
Anti-Acetyllysine Mouse mAb	PTM Biolabs	Cat# PTM-101
Chemicals, peptides, and recombinant proteins
H3-20 peptide: Ac-ARTKQTARKSTGGKAPRKQL	This paper	N/A
H4-20 peptide: Ac-SGRGKGGKGLGKGGAKRHRK	This paper	N/A
Benzoyl coenzyme A lithium salt	Sigma-Aldrich	Cat# B1638
Acetyl coenzyme A lithium salt	Sigma-Aldrich	Cat# A2181
Critical commercial assays
lipofectamine 2000	Invitrogen	Cat# 11668019
Deposited data
Proteomics dataset of the Kbz sites in the quantitative benzoylome	PRIDE	PXD034187
Experimental models: Cell lines
293T	NCACC	Cat# GNHu17
HepG2	NCACC	Cat# TCHu72
Hela	NCACC	Cat# TCHu187
Recombinant DNA
Plasmid: pCMV-Flag-HAT1	This paper	N/A
Plasmid: pCMV-Flag-CBP	This paper	N/A
Plasmid: pCMV-Myc-p300	This paper	N/A
Plasmid: pCMV-Flag-GCN5	This paper	N/A
Plasmid: pCMV-Flag-MOF	This paper	N/A
Plasmid: pCMV-Flag-PACF	This paper	N/A
Plasmid: pCMV-Flag-Tip60	This paper	N/A
Plasmid: pCMV-Flag-HBO1	This paper	N/A
Plasmid: pCMV-Flag-HBO1 G485A/E508Q	This paper	N/A
Plasmid: pCMV-Flag-JADE1	This paper	N/A
Software and algorithms
MaxQuant software (v1.6.15.0)	MaxQuant	https://www.maxquant.org/
PyMol package	Pymol	http://www.pymol.org/
R clusterProfiler package	R-project	https://www.r-project.org/
iceLogo (v1.2)	IceLogo	https://iomics.ugent.be/icelogoserver/
ImageJ	National Institutes of Health	https://imagej.nih.gov/ij/