Figure 15.

In-vitro cell viability assay of synthesized compounds on the fibroblasts cell line. At 5 × 104 cells/well cell density, cells were treated with several drug concentrations (0.1, 1, 10, and 100 µM) of investigated compounds or positive comparator, ibuprofen, for 24 h exposure time. Cytotoxicity was identified via MTT-based colorimetric assay (450 nm), and % cell growth viability was estimated in triplicates, showing HI and HL as active derivatives relative to ibuprofen as standard control.