Figure 5.

(A) Treatment with 15o (2 µM) and 15r (1 µM) in HT-29 cells caused the increased formation of JC-1 aggregates when compared to control cells, while a significant reduction in the levels of JC-1 monomers was found in the treated groups. (B) Compounds 15o (2 µM) and 15r (1 µM) induced apoptosis in HT-29 colon cancer cells. Flow cytometry graphs depicting early apoptosis, late apoptosis, and necrotic population of HT-29 cells. (C) Flow cytometry bar graph depicting the% JC-1 cell population of HT-29 cells. (D) Bar graphs representing early and late apoptotic cell populations in HT-29 cells observed after Annexin/PI staining. Statistical significance was determined by two-way ANOVA followed by Tukey’s post hoc analysis, where *** p < 0.001 represents control vs. 1,2,4-triazolo-linked bis-indolyl conjugates (15o and 15r) in the treatment groups.