. 2022 Oct 31;11(21):3456. doi: 10.3390/foods11213456

Table 10.

Molecular techniques commonly used for AAB identification.

Method	Microorganism	Primer Sequence (5′–3′)	Reference
16S rRNA, Sanger sequencing	Gluconacetobacter;	27F, AGAGTTTGATCMTGGCTCAG	[107]
	Komagataeibacter;	1494R, TGACTGACTGAGGYTACCTTGTTACGACTT
	Gluconacetobacter;	fD1, CCGAATTCGTCGACAACAGAGTTTGATCCTGGCTCAG	[29]
	kombuchae sp. nov.;	rD1, CCCGGGATCCAAGCTTAAGGAGGTGATCCAGCC
	Acetobacter aceti;	16S d, GCTGGCGGCATGCTTAACACA	[96]
	Acetobacter aceti;	16S r, GCAGGTGATCCAGCCGCA	[96]
16S rRNA V1-V3 region, Illumina MiSeq	Bacterial communities	Forward, TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG	[108]
16S rRNA V1-V3 region, Illumina MiSeq	Bacterial communities	Reverse, GTVTVGTGGGCTCGGAGATGTGTATAAGAGACAG	[108]
16S rRNA V3-V4 region, Illumina MiSeq	Bacterial communities	Forward, CCTACGGGNGGCWGCAG	[102] [109]
16S rRNA V3-V4 region, Illumina MiSeq	Bacterial communities	Reverse, GACTACHVGGGTATCTAATCC	[102] [109]
16S rRNA, Real-time PCR	Bacterial abundance	926f, AAACTCAAKGAATTGACGG	[100]
16S rRNA, Real-time PCR	Bacterial abundance	1062r, CTCACRRCACGAGCTGAC	[100]
16S rRNA, T-RFLP	Bacterial communities	27F, AGAGTTTGATCMTGGCTCAG	[17]
16S rRNA, T-RFLP	Bacterial communities	1525R, AAGGAGGTGATCCAGCC	[17]
RAPD	Komagataeibacter spp.	M13, GAGGGTGGCGGTTCT	[104]
AFLP	Komagataeibacter rhaeticus	A03, GACTGCGTACAGGCCCCG	[110]
AFLP	Komagataeibacter rhaeticus	T03, CGATGAGTCCTGACCGAG	[110]
REP-PCR	G. oxydans; A. aceti	REPIR-I, IIICGICGICATCIGGC	[105]
REP-PCR	G. oxydans; A. aceti	REP2-I, ICGICTTATCIGGCCTAC	[105]
ERIC-PCR	G. oxydans; A. aceti	ERIC1R, ATGTAAGCTCCTGGGGATTCAC	[105]
ERIC-PCR	G. oxydans; A. aceti	ERIC2, AAGTAAGTGACTGGGGTGAGCG	[105]
Shotgun metagenomic sequencing	Bacterial and fungal communities		[101] [102]