Figure 1.

Compressive force increases global histone lysine acetylation further augmented by oleic acid exposure. (a, b) Quantitative expression analysis of genes encoding histone acetyl transferases TAF1, EP300 and CREBBP (a) and encoding histone deacetylases HDAC1, HDAC2 and HDAC3 (b) in human periodontal ligament fibroblasts (HPdLFs) exposed to oleic acid (OA) and stimulated with compressive force (CF) compared to BSA controls. (c, d) Analysis of global H3 lysine acetylation level (H3Kac) presented in representative microphotographs of HPdLFs treated with OA and stimulated with CF in comparison to BSA controls (c) and analysed in (d) in relation to BSA control as percent changes. DAPI labels the cell nuclei and H3Kac staining is shown in thermal LUT (H3Kac th. LUT), indicating staining intensity. The dashed lines illustrate the positions of the cell nuclei. *** p < 0.001 in relation to BSA, ### p < 0.001 in relation to PA, §§§ p < 0.001 in relation to BSA + CF; One-Way ANOVA and post hoc test (Tukey). Scale bars: 10 μm in (c). n.s., not significant; RNE, relative normalized expression.