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. 2022 Jun 28;17(13):1892–1904. doi: 10.1080/15592294.2022.2090654

Table 2.

qPCR primer sequences of human IL10 in promoter regions (#1, #2, #3) and a non-promoter region (#4) indicated in 5`-3` direction. bp, base pairs; fw, forward; Length, amplicon length; rev, reverse.

Location Label Primer Sequence Length
promoter region #1 fw: TGAAGAAGTCCTGATGTCACrev: TTACCTATCCCTACTTCCCC 187 bp
promoter region #2 fw: AGCACTACCTGACTAGCATArev: AGAGACTGGCTTCCTACAG 192 bp
promoter region #3 fw: GGGGACCCAATTATTTCTCArev: TGGGCTACCTCTCTTAGAAT 188 bp
non-promoter region #4 fw: GCTTAGAGCGTTTCCAGACCrev: CTCCCCACTGTAGACATCCA 131 bp