Table 3. Sequence kernel association tests of low frequency and rare ENaC variants with blood pressure measures.

SKAT was performed on variants with a MAF < 0.05 in the genomic regions of SCNN1A, SCNN1B, SCNN1D, and SCNN1G with SBP, DBP, PP, and MAP, separately, and then combined (SCNN1A, SCNN1B and SCNN1G). SKAT was separately performed on rare (MAF < 0.01) and low frequency (0.01 ≤ MAF < 0.05) variants. Significant p values are in bold.

Gene. Chromosome:Start–End		Variants with MAF < 0.05		Variants with MAF < 0.01		Variants with 0.01 ≤ MAF < 0.05
	Measure	n	p value	n	p value	n	p value
SCNN1A. 12:6346824–6379751	SBP	3,972	0.02	3,859	0.03	113	0.07
	DBP	3,972	0.00004	3,859	0.0004	113	0.0007
	PP	3,972	0.7	3,859	0.3	113	0.9
	MAP	3,972	0.0002	3,859	0.002	113	0.002
SCNN1B. 16:2329812–23381320	SBP	12,334	0.02	11,925	0.04	409	0.04
	DBP	12,334	0.002	11,925	0.003	409	0.01
	PP	12,334	0.3	11,925	0.3	409	0.04
	MAP	12,334	0.003	11,925	0.006	409	0.01
SCNN1D. 1:1280417–1292050	SBP	2,405	0.0008	2,293	0.2	112	0.0005
	DBP	2,405	0.002	2,293	0.03	112	0.003
	PP	2,405	0.003	2,293	0.04	112	0.005
	MAP	2,405	0.0008	2,293	0.1	112	0.0007
SCNN1G. 16:23184696–23216904	SBP	4,093	0.5	4,022	0.3	71	0.6
	DBP	4,093	0.2	4,022	0.1	71	0.4
	PP	4,093	0.06	4,022	0.1	71	0.09
	MAP	4,093	0.5	4,022	0.2	71	0.8
Combined (SCNN1A, SCNN1B, SCNN1G)	SBP	20,399	0.01	19,806	0.02	593	0.03
	DBP	20,399	0.0002	19,806	0.0004	593	0.004
	PP	20,399	0.3	19,806	0.1	593	0.4
	MAP	20,399	0.0006	19,806	0.001	593	0.005
Combined (SCNN1A, SCNN1B, SCNN1D, SCNN1G)	SBP			22,099	0.01	705	0.006
	DBP			22,099	0.0002	705	0.001
	PP			22,099	0.08	705	0.14
	MAP			22,099	0.0009	705	0.001

^*p values < 0.00625 are significant