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. Author manuscript; available in PMC: 2023 May 1.
Published in final edited form as: Oncogene. 2022 Oct 12;41(46):5020–5031. doi: 10.1038/s41388-022-02491-8

Fig. 4. PARPi-resistant PEO1 cell lines slow fork progression through increasing unscheduled origin firing.

Fig. 4.

(a) DNA fiber assays were performed to study alterations in replication fork dynamics, as described in Materials and Methods. The schematics are shown above. Average replication fork speeds (bottom left) and inter-origin distances (bottom right) in each group are shown. (b) Cells were treated with or without CHK1 inhibitor (CHK1i) prexasertib and its effect on DNA damage quantitated by comet assay (left), fork stabilization assayed by DNA fiber assay (middle), and HR repair assessed by RAD51 foci (right). *, p < 0.05; ***, p < 0.001; ns, not significant.