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. 2022 Oct 24;12(17):7476–7490. doi: 10.7150/thno.75421

Figure 5.

Figure 5

SIAIS361034 retards hair growth in a depilatory model by inhibiting the Hh pathway activity. (A) Plasma concentrations of SIAIS361034 after a single 2 mg/kg intravenous (i.v.) injection, 10 mg/kg intraperitoneal (i.p.) injection or 10 mg/kg oral (p.o.) administration. Data represent mean ± SD (n = 3). (B) Illustration of the experimental design of depilatory model. This model image was created in BioRender.com. (C) RT-qPCR was carried out to determine Gli1 expression in the skin samples from mice on indicated days after depilatory treatment. Data represent mean ± SD (n = 3). (D) The gross phenotype of the back skin of the mice during the administration period. Pictures of mice are shown (n = 4). (E) The depilatory model mice were sacrificed for skin tissues 6 h after the last drug application. RT-qPCR was carried out to determine Gli1 expression in the skin samples from mice. Data represent mean ± SD (n = 4). (F) A representative western blot analysis of Gli1 and Bcl-xL in the skin samples from the mice at the end of the experiment (n = 4). β-tubulin was used as a protein loading control.