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SDS-PAGE analyses of the reaction solutions of the auto-processing of the cassette mutated [H56A]-proCCN’ (a), [D99A]-proCCN’ (b), and [S193A]-proCCN’ (c) proteins. Lanes 1, 2, and 3: purified proCCN’, treated with enterokinase, and subsequently treated with the EKaptureTM agarose, respectively. Black and red arrowheads indicate proCCN and CCN protein, respectively.
