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. 2022 Nov 20;27(22):8063. doi: 10.3390/molecules27228063

Table 1.

The Km and Vmax values of the mutant proteins.

Recombinant Protein Km
(M)		 Vmax
(μmol/L/s)
Wild type CCN 6.4 × 10−4 4.5 × 10−4
[K63G]-CCN 4.0 × 10−4 1.0 × 10−4
[K63G,K131G,K133A]-CCN (CCN’) 3.7 × 10−4 1.4 × 10−4
The mature CCN’ derived from
[K8D,K63G,K131G,K133A]-proCCN		 9.2 × 10−4 4.6 × 10−4

The enzyme activity of the wild type is cited from a previous report [8]. The Km and Vmax values were calculated by fitting the experimental data points to a Lineweaver–Burk plot.