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Concentration levels of independent variables and response constraints of dependent variables.
| Independent Variables | Lower Level | Upper Level | |
|---|---|---|---|
| Factor 1 (A+B) | A = solid lipid | 65% | 95% |
| B = liquid lipid | 5% | 35% | |
| Factor 2 | C = surfactant | 2% | 5% |
| Dependent variables | Constraints | ||
| Response 1 | Particle size (nm) | Minimum | |
| Response 2 | PDI | In range (0.2–0.25) | |
| Response 3 | Entrapment efficiency (%) | In range (85–95%) | |
