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. 2022 Nov 7;11(11):1305. doi: 10.3390/pathogens11111305

Figure 1.

Figure 1

PCR results for Neospora caninum (left) and Toxoplasma gondii (right) DNA. Seropositive bulk milk samples and seronegative bulk milk samples containing a number of positive individual samples were analyzed by PCR (lanes 1, 7, 10, 11, 12, and 13, indicating the ID of the tested bulk milk samples). A fragment of Nc5 gene (337 bp) of N. caninum and B1 gene (196 bp) of T. gondii was amplified, and the product was run on 1.5% agarose gel. Bulk 1 was positive for N. caninum, and bulks 10, 12, and 13 were positive for T. gondii. Lane M: 100 bp DNA ladder. Lane P: positive control.