Table 5.

Reactive flavivirus ELISA results from raccoon blood samples where no differentiation was possible between WNV, USUV and TBEV by neutralization assays. Samples were considered positive (POS) by blocking ELISA when the inhibition percentage (IP) was ≥40%, doubtful (DOUBT) when IP > 30% to <40% and negative when IP ≤ 30% (not shown). Positive ELISA and neutralization assay results are shown in bold. As no differentiation was possible via neutralization assays, these serum samples from the raccoons were excluded from the calculation of seroprevalences and were classified as an infection by an undetermined flavivirus or by multiple flaviviruses.

	Blocking ELISA		Differentiation by Virus Neutralization Tests
FLI ID-Number	Inhibition Percentage (IP%)	Result	WNV Antibody Titer (ND50)	USUV Antibody Titer (ND50)	TBEV Antibody Titer (ND50)
1-W-B-125	47.27	POS	480	80	160
1-M-B-002	43.17	POS	<10	30	10
1-W-B-023	32.61	DOUBT	<10	<10	<10
1-W-B-034	32.04	DOUBT	<10	20	10
1-W-B-120	33.55	DOUBT	30	60	<10
1-W-B-179	88.90	POS	80	30	<10
1-W-B-185	40.24	POS	40	30	<10