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. 2022;2434:257–264. doi: 10.1007/978-1-0716-2010-6_17

Establishment of In Vitro Brain Models for AON Delivery.

Elena Daoutsali, Ronald A M Buijsen
PMCID: PMC9703237  PMID: 35213023

Abstract

Progress in stem cell biology has made it possible to generate human-induced pluripotent stem cells (hiPSC) that can be differentiated into complex, three-dimensional structures, where the cells are spatially organized. To study brain development, Lancaster and colleagues developed an hiPSC-derived three-dimensional organoid culture system, termed cerebral organoids, that develop various discrete, although interdependent, brain regions. Here we describe in detail the generation of cerebral organoids using a modified version of the culture protocol.

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