Corrigendum: Striatal dopamine D2-muscarinic acetylcholine M1 receptor-receptor interaction in a model of movement disorders

In the published article, there was an error in Figure 1A as published. The immunoblot panel corresponding to the Lysates/IB: anti-FLAG (upper left corner) was erroneously swapped with the immunoblot panel of IPs: anti-HA/IB: anti-HA (lower right corner). The corrected Figure 1 and its caption appear below.

FIGURE 1.

D₂R-M₁R interaction in transiently transfected HEK293T cells. (A) Co-immunoprecipitation. HEK293T cells were harvested and lysed 48 h after transfection. The lysates were used for immunoblotting (IB) with anti-FLAG and anti-HA antibodies to demonstrate D₂R and M₁R expression, respectively (left panels). The rest of the samples (immunoprecipitates; IPs) were subjected to immunoprecipitation with a mouse anti-HA antibody. The CoIP was confirmed via the detection of FLAG-D₂R upon IB with rabbit anti-FLAG and rabbit anti-HA antibodies (right panel; boxed lane). Data shown are representative of three independent experiments. (B) BRET¹ saturation curve. The BRET¹ signal in HEK293T cells co-expressing a constant amount of D₂R-Rluc and increasing amounts of M₁R-YFP (n = 5) or YFP (n = 3) constructs was measured 48 h post-transfection. The BRET¹ saturation curve is derived from all independent experiments. (C) NanoBiT^® complementation assay. The SmBiT and LgBiT parts of the NanoLuciferase fragments were fused to the C-terminus of the indicated receptor. The constructs were overexpressed via transient transfection in HEK293T cells. Results are presented as mean ± SD (n = 3). Statistical significance was tested using the nonparametric ANOVA by ranks of Kruskal–Wallis followed by the Dunn’s multiple comparisons post-hoc test, * = p ≤ 0.05.

The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.

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