Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Anti-PC1/Pcsk1 (rabbit polyclonal) |
Abcam | Cat# ab3532, RRID:AB_303882 | 1:750 dilution |
| Antibody | Anti-CGRP (rabbit polyclonal) | Sigma | Cat# C8198, RRID:AB_259091 | 1:500 dilution |
| Antibody | Anti-POMC (rabbit polyclonal) | Phoenix Pharmaceuticals | Cat# H-029–30 | 1:500 dilution |
| Antibody | Anti-CARTPT (rabbit polyclonal) | Phoenix Pharmaceuticals | Cat# H-003–62 | 1:2000 dilution |
| Antibody | APC–conjugated anti-mouse CD31 (mouse monoclonal) | BioLegend | Cat# 102409 | 1:800 dilution |
| Antibody | APC-anti-mouse CD45 (mouse monoclonal) | BioLegend | Cat# 103111 | 1:800 dilution |
| Antibody | APC-anti-mouse F4/80 (mouse monoclonal) | BioLegend | Cat# 123115, clone BM8 | 1:800 dilution |
| Antibody | PE-Cy7-conjugated anti-mouse CD326 (mouse monoclonal) | Thermo Fisher Scientific | Cat# 25-5791-80, clone G8.8 | 1:400 dilution |
| Antibody | Anti-IgG A488 (goat polyclonal) | Invitrogen | Cat# A11034 | 1:250 dilution |
| Antibody | Anti-IgG A555 (goat polyclonal) | Invitrogen | Cat# A21229 | 1:250 dilution |
| Antibody | Anti-IgG A633 (goat polyclonal) | Invitrogen | Cat# | 1:250 dilution |
| Antibody | Anti-rat IgG (donkey polyclonal) | Jackson ImmunoResearch | Cat# 712-605-153 | 1:250 dilution |
| Biological sample (Homo sapiens, male and female) | Lung tissue | Stanford University | NA | NA |
| Biological sample (H. sapiens, female) | Human pulmonary carcinoid tissue | Stanford University | NA | NA |
| Strain, strain background (Mus musculus, male and female) | Wild type: CD-1 and C57BL/6NJ | Charles River | Strain Code 022, Strain Code 027 |
NA |
| Strain, strain background (M. musculus, male and female) | Ascl1 CreERT2 |
Kim et al., 2011a. PMID:21483754 |
NA | NA |
| Strain, strain background (M. musculus, male and female) | Agtr1a-Cre; B6(C3)-Agtr1atm1.1(cre)Ekrs/J | The Jackson Laboratory | JAX: 030553 | NA |
| Strain, strain background (M. musculus, male and female) | Agtr1a-2A-Cre; B6.FVB-Agtr1aem1(cre)Zak/J | The Jackson Laboratory | JAX: 031487 | NA |
| Strain, strain background (M. musculus, male and female) | Calb1-2A-dgCre: B6.Cg-Calb1tm1.1(folA/cre)Hze | The Jackson Laboratory | JAX: 023531 | NA |
| Strain, strain background (M. musculus, male and female) | Mouse: Rosa26Zsgreen1, B6.Cg-Gt(ROSA)26Sortm6(CAG-ZsGreen1)Hze/J | Madisen et al., 2009 | Stock No: 007906 | NA |
| Commercial assay or kit | RNAscope Hiplex in situ hybridization assay | Advanced Cell Diagnostics (ACD) | Cat#324140 | NA |
| Commercial assay or kit | RNAscope Hiplex assay probe Mm-Agt-T1 | Advanced Cell Diagnostics (ACD) | Cat#426941-T1 | NA |
| Commercial assay or kit | RNAscope Hiplex assay probe Mm-Nmb-T2 | Advanced Cell Diagnostics (ACD) | Cat#459931-T2 | NA |
| Commercial assay or kit | RNAscope Hiplex assay probe Mm-Adcyap1-T3 | Advanced Cell Diagnostics (ACD) | Cat#405911-T3 | NA |
| Commercial assay or kit | RNAscope Hiplex assay probe Mm-Cartpt-T5 | Advanced Cell Diagnostics (ACD) | Cat#432001-T5 | NA |
| Commercial assay or kit | RNAscope Hiplex assay probe Mm-Pomc-T6 | Advanced Cell Diagnostics (ACD) | Cat#314081-T6 | NA |
| Commercial assay or kit | RNAscope Hiplex assay probe Mm-Chga-T9 | Advanced Cell Diagnostics (ACD) | Cat#447851-T9 | NA |
| Commercial assay or kit | RNAscope Hiplex assay probe Mm-Calca-T7 | Advanced Cell Diagnostics (ACD) | Custom probe | NA |
| Commercial assay or kit | RNAscope Hiplex assay probe Mm-Scg5-T10 | Advanced Cell Diagnostics (ACD) | Custom probe | NA |
| Software, algorithm | Integrative Genomics Viewer (IGV v2.4.14) |
Katz et al., 2010. PMID:21057496 |
https://software.broadinstitute.org/software/igv/ | NA |
| Software, algorithm | Seurat v2.3.4 |
Butler et al., 2018. PMID:29608179 |
https://satijalab.org/seurat/ | NA |