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. 2022 Nov 23;14(23):4983. doi: 10.3390/nu14234983

Figure 1.

Figure 1

Dietary emulsifiers exacerbated food allergy-induced parameters of anaphylaxis. (A) Experimental flow chart: three-week-old BALB/c mice were treated with Water, CMC, or P80 for three months. After six weeks of exposure to Water, CMC, or P80, mice were sensitized with intraperitoneal injection with 20 mg of OVA and Alum, followed by another sensitization in two weeks. After another two weeks, the mice were orally administered 50 mg of ovalbumin five times. Mice were euthanized at the end of experiments and used for subsequent analysis. (B) Time course of hypothermia. Rectal temperature was evaluated just after the final oral challenge with OVA (n = 5, Control; n = 10, Water; n = 9, CMC; n = 10, P80). (C) Diarrhea scores. The extent of diarrhea was examined just after the final oral challenge with OVA (n = 17, Water; n = 18, CMC; n = 19, P80). (D) OVA-specific IgE in plasma collected from food allergy-induced mice was measured by ELISA (n = 10 per group). One-way ANOVA and Tukey’s Multiple Comparison Test were used to determine significance. Data are the means ± SEM. * p < 0.05, ** p < 0.01; ns, not significant.