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. 2022 Dec 2;27(23):8475. doi: 10.3390/molecules27238475

Figure 4.

Figure 4

Resveratrol treatment reversed the accumulation of P62 to enhance autophagy levels and attenuate fibrosis. (A) The mRNA level of LC3B was quantified. (B,C) The correlation between LC3B and FN1 and COL3A1 gene expression showed a stronger positive correlation. Spearman’s r and p values are indicated. (D) Levels of SOD2, HIF-1a, and LC3 in lung tissues were detected using Western blotting. (E) The immunoblotting data of LC3 II/I were quantified as described above. (F,G) The mRNA levels of P62 and BNIP3 were quantified. (H) The correlation between P62 and FN1 gene expression showed a stronger positive correlation. Spearman’s r and p values are indicated. (I) Levels of P62, p-P62(phospho-SQSTM1/p62(Ser349)), BNIP3, BECN1, and BCL2 in lung tissues were detected using Western blotting. (J) The relative intensity of P62 was quantified as described above using Western blotting. Values represent the mean ± SEM (n = 3–5). * p < 0.05 and ** p < 0.01 compared with the control group, and compared with the model group (CIA+BLM), # p < 0.05 and ## p < 0.01.