Skip to main content
. 2022 Dec 12;25(3):330–340. doi: 10.1016/j.jcyt.2022.12.001

Figure 5.

Figure 5

Effect of dexamethasone and IL-15 on total PBMCs and CD45RA− memory T-cell proliferation. PBMCs were cultured in presence or absence of IL-15 (50 ng/mL) O/N. Then, cells were washed and labeled with CFSE and incubated for 72 h with different concentrations of dexamethasone (0, 10−7 mol/L DEX, 10−6 mol/L DEX and 10−5 mol/L) for 72 h. (A) Proliferation Index of total live cells and CD45RA− memory T cells. The PI is calculated as total number of divisions divided by the number of cells that went into division. One-way analysis of variance was performed to analyze differences due to different concentrations of dexamethasone previously cultured with IL-15 O/N. Mean ± SEM, N = 5–9 independent donors. (B) Representative determination of live cells and CD45RA− memory T cells proliferation by flow cytometry with or without IL-15 and in presence of increasing concentrations of dexamethasone. (C) Geometric mean fluorescence intensity (GeoMFI), expressed as ratio GeoMFI /(GeoMFI of the negative control) (cells without dexamethasone and without IL-15). Mean ± SEM of the ratios are represented. SEM, standard error of the mean.