Abstract
During intraoperative fluorescence navigation to remove various neoplasms and during pharmacokinetic studies of photosensitizers in laboratory animals, in many cases, the ratio of photosensitizer accumulation in the tumor and normal tissue can reach ⩾ 10-fold, which inevitably changes their optical properties. At the same time, the tumor formation process causes various metabolic and structural changes at cellular and tissue levels, which lead to changes in optical properties. A hardware-software complex for the spectral-fluorescence studies of the content of fluorochromes in biological tissues with significantly different optical properties was developed, and it was tested on optical phantoms with various concentrations of photosensitizers, absorbers, and scatterers. To correct the influence of optical properties on the photosensitizer concentration analysis by fluorescence spectroscopy, we propose the spectrum-processing algorithm, which combines empirical and theory-based approaches.
Keywords: fluorescence, spectroscopy, scattering, absorption, attenuation correction, optical phantoms
Acknowledgements
The reported study was funded by Russian Foundation for Basic Research (RFBR) according to the research project No. 18-29-01062.
Footnotes
Tatiana A. Savelieva received her Master of Engineering degree with a specialization in Engineering in biomedical practice in 2005 and Ph.D. degree in Laser Physics in 2013 from A.M. Prokhorov General Physics Institute of the Russian Academy of Sciences, Russia.
Now Tatiana A. Savelieva is working as a researcher at General Physics Institute and an associate professor at National Research Nuclear University MEPhI, Russia. The area of scientific interests includes biophotonics and various methods of optical spectroscopy applied to the differentiation of biological tissues.
Marina N. Kuryanova received her Master’s degree in Nuclear Physics and Technology from National Research Nuclear University MEPhI, Russia. She completed her pre-graduate practice at Department of Laser, Micro and Nanosystems. Recently, she was engaged in solving the problem of improving the accuracy of fluorescence diagnostics in conditions of significantly different optical properties of the studied organs and tissues. Solving this problem will increase the effectiveness of diagnostics and determining the boundaries of tumors.
Ekaterina V. Akhlyustina studied at National Research Nuclear University MEPhI, Russia, in 2011–2015 at the department No. 35 “Medical Physics”, the direction of training “Radiation Biophysics”, a diploma was written on the topic “The effect of different doses of gamma radiation on neurogenesis in the dentate fascia of the hippocampus and on the spatial behavior of adult mice” (head Lazutkin A.A.). In 2015–2017, she studied at National Research Nuclear University MEPhI at the department No. 87 “Laser micro-, nano- and biotechnologies”, the direction of training was “Biomedical Photonics”. From 2017 to present time, she is postgraduate student at National Research Nuclear University MEPhI, direction of training “Physics and Astronomy”.
Kirill G. Linkov received his Master of Engineering degree with a specialization in Electronic Devices and Equipment (Electronics and optoelectronic engineering dept.) in 1994 and Ph.D. degree in Engineering with a specialization in Quantum Electronics in 1999 from Moscow Institute of Radio Engineering, Electronics and Automation, Russia.
Kirill G. Linkov is Senior Scientist at Prokhorov General Physics Institute of the Russian Academy of Sciences, Russia, and engages in development of methods for laser-fluorescent diagnostics, and photodynamic therapy. He takes part as Investigator or Principal Investigator in the research projects that are being implemented with financial support from Russian Foundation for Basic Research (RFBR), Russian State Atomic Energy Corporation “Rosatom”, Ministry of Education and Science of the Russian Federation, Russia. Kirill G. Linkov has presented in various professional conferences and has more than 40 published works including publications in peer-reviewed journals, such as Laser Physics, Lasers in Medical Science, Methods and Applications in Fluorescence, Biomedical Photonics. He is also the author or co-author of more than 20 patents.
Gennady A. Meerovich is Senior Scientist at Prokhorov General Physics Institute of the Russian Academy of Sciences, associate professor at National Research Nuclear University MEPhI, Russia. He received the degree of “Candidate of Physical and Mathematical Sciences”, eq. to the international Ph.D. degree, in 1981 with topic of PhD Thesis: “Investigation and optimization of active elements of scanning Semiconductor Electron-Beam-Pumped Lasers”. From 1994 till present, he is working as senior researcher in Laser Biospectroscopy Laboratory (Center for Nature Science Investigations, General Physics Institute of the Russian Academy of Sciences, Moscow, Russia) on research and development of new methods, devices and photosensitizers for photodynamic treatment and fluorescent diagnostics of cancer. Novel lasers and laser systems, scanning laser systems, computerassisted system for diagnostics are also in the field of his scientific interests.
Victor B. Loschenov is professor and head of laboratory at Prokhorov General Physics Institute of the Russian Academy of Sciences, professor at National Research Nuclear University (NRNU) MEPhI, Russia. His research interests are focused on fundamentals and practical applications of photodynamic therapy and fluorescent diagnostics of cancer, medical devices, phototheranostics, different modalities of optical spectroscopy. He received the Ph.D. degree in 1981 from N.S. Kurnakov Institute of General and Inorganic Chemistry, Academy of Sciences of the USSR on specialty “Quantum radio physics”. In 2006, he received the degree of doctor of science in laser physics from Prokhorov General Physics Institute of the Russian Academy of Sciences with subject: “Methods and apparatus for spectral-fluorescence diagnosis and photodynamic therapy”. Since 2007, he has a title of professor in Laser physics. His professional experience includes the position of head of Laser Biospectroscopy Laboratory at GPI RAS (Moscow, Russia) (from 1989 till present) and professor and head of department in NRNU MEPHI, Russia.
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