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. 2020 Jul 10;13(4):307–317. doi: 10.1007/s12200-020-1040-0

In vivo volumetric monitoring of revascularization of traumatized skin using extended depth-of-field photoacoustic microscopy

Zhongwen Cheng 1, Haigang Ma 2, Zhiyang Wang 2, Sihua Yang 1,2,✉
PMCID: PMC9743921  PMID: 36641563

Abstract

Faster and better wound healing is a critical medical issue. Because the repair process of wounds is closely related to revascularization, accurate early assessment and postoperative monitoring are very important for establishing an optimal treatment plan. Herein, we present an extended depth-of-field photoacoustic microscopy system (E-DOF-PAM) that can achieve a constant spatial resolution and relatively uniform excitation efficiency over a long axial range. The superior performance of the system was verified by phantom and in vivo experiments. Furthermore, the system was applied to the imaging of normal and trauma sites of volunteers, and the experimental results accurately revealed the morphological differences between the normal and traumatized skin of the epidermis and dermis. These results demonstrated that the E-DOF-PAM is a powerful tool for observing and understanding the pathophysiology of cutaneous wound healing.

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Keywords: photoacoustic microscopy (PAM), extended depth-of-field, traumatized skin

Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant Nos. 61822505, 11774101, 61627827, and 81630046), the Science and Technology Planning Project of Guangdong Province, China (No. 2015B020233016), and the Science and Technology Program of Guangzhou (No. 2019050001).

Footnotes

Zhongwen Cheng is a Ph.D. candidate student from College of Biophotonics, South China Normal University, China. His research interests includes photoacoustic microscopy and photoacoustic tomography.

Haigang Ma is a Ph.D. candidate student from College of Biophotonics, South China Normal University, China. His research interests includes utilizing photoacoustic imaging for detection of skin diseases and clinical applications.

Zhiyang Wang is a Ph.D. candidate student from College of Biophotonics, South China Normal University, China. His research interests focus on photoacoustic microscopy and its clinical applications.

Sihua Yang is a professor at MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, China. His research focuses on photoacoustic imaging, photoacoustic endoscopic technology, instrumentation and their biomedical applications.

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