Fig. 2. FTO regulates LINE1 RNA level through m⁶A demethylation.

(A) m⁶A peak profiles on m⁶A-marked LINE1 RNA in WT and Fto^−/− mESCs. (B) Volcano plot showing differentially expressed subfamilies of m⁶A-marked repeat RNAs upon Fto KO. (C) Cumulative distribution and boxplots (inset) showing nuclear LINE1 RNA lifetime in WT and Fto^−/− mESCs. P value was determined using Wilcoxon signed-rank test. (D) Relative enrichment of LINE1 RNA by YTHDC1 from cross-linking and immunoprecipitation quantitative polymerase chain reaction (qPCR) in WT and Fto^−/− mESCs. P values were determined using unpaired two-tailed t tests. Error bars and means ± SD are shown for n = 3 experiments. (E) Cumulative distribution and boxplots (inset) showing LINE1 RNA transcription rate in WT and Fto^−/− mESCs. P value was determined using Wilcoxon signed-rank test. (F) Cumulative distribution and boxplots (inset) showing the difference in transcription rate changes between m⁶A-marked and -unmarked LINE1 RNA upon Fto KO. P value was determined using Wilcoxon rank sum test.