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. 2022 Dec 14;14(12):e32519. doi: 10.7759/cureus.32519

Table 1. Primer sequences for a real-time PCR assay.

HO-1, heme oxygenase-1; Nrf2, nuclear factor erythroid 2-related factor 2; TNF-α, tumor necrosis factor-α; TNFR1, TNF-α type 1 receptor

Gene Primer pair sequences Gene ID
TNF-α F: 5`-AAATGGGCTCCCTCTCATCAGTTC-3` X66539
R: 5`-TCTGCTTGGTGGTTTGCTACGAC-3`
TNFR1 F: 5`-CCAAGTGCCACAAAGGAACC-3` NM_013091.2
R: 5`-TCAGGTAGCGCTGGAATTGG-3`
Nrf2 F: 5`-AGCAGGACATGGATTTGATT-3` XM_032903520.1
R: 5`-CTTCTCCTGTTCCTTCTGGA-3`
HO-1 F: 5`-ACAGAAGAGGCTAAGACCG-3` XM_032887931.1
R: 5`-CAGGCATCTCCTTCCATT-3`
GAPDH F: 5′-CCATCAACGACCCCTTCATT-3′ NM_017008
R: 5′-CACGACATACTCAGCACCAGC-3′