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. 2022 Dec 15;17(12):e0278897. doi: 10.1371/journal.pone.0278897

Further evidence in support of the validity of the post-secondary student stressors index using a nationwide, cross-sectional sample of Canadian university students

Brooke Linden 1,2,*, Heather Stuart 1,2
Editor: Supat Chupradit3
PMCID: PMC9754283  PMID: 36520875

Abstract

The Post-Secondary Student Stressors Index (PSSI) was created to facilitate improved evaluation of the sources of post-secondary student stress. This study reports evidence in support of the validity of the tool using a large, nationwide cross-sectional sample of students attending universities across Canada during the 2020–2021 academic year. We provide additional evidence for the construct validation of the PSSI, including internal structure evidence and relations to other variables by estimating multiple-indicator, multiple-cause models and investigating Spearman’s rho correlation coefficients between the PSSI and like constructs. Based on index validation guidelines, results provide further support for the internal structure of the PSSI, demonstrating hypothesized relationships with like constructs and manifest variables, as well as acceptable goodness-of-fit statistics. Similarly, correlation coefficients were statistically significant and in line with directionality hypotheses. The results of this research provide further evidence for the validity of the PSSI among varied university student populations in Canada and addresses several of the limitations identified in earlier preliminary psychometric work on the instrument.

Introduction

Although the prevalence of mental illnesses among post-secondary students are not significantly different from those observed among the general Canadian population [1], the demand for mental health services on post-secondary campuses continues to grow as does the complexity of presenting issues [2]. National cross-sectional data suggests that the prevalence of students reporting above average to extreme levels of stress is increasing, along with self-reported symptoms of common mental illnesses such as anxiety and depression [3]. Chronic stress has been linked to poor academic performance and negative mental health outcomes [4], necessitating the bolstering of upstream campus services that provide students with the tools needed to recognize and respond to changes in their mental health in the face of day-to-day stressors. In order to appropriately tailor these upstream services to the needs of students, a method of measuring the sources of student stress is required.

Until recently, there has been no validated measurement tool for assessing the frequency and severity of stressors experienced by students within post-secondary settings. Existing instruments designed to evaluate student stress are associated with a host of issues, including being conceptualized too broadly or too narrowly, seldom involving students in the development process (often focusing on a single year or program), or demonstrating a lack of strong psychometric properties. These instruments are also often outdated, with the majority having been developed between 1990 and 2005. This raises concerns regarding the validity of the instruments with respect to accurate and comprehensive measurement of the stressors that are currently salient for students [5].

In 2019, the Post-Secondary Student Stressors Index (PSSI) was developed in response to these gaps in measurement. The tool was developed and validated with extensive collaboration with students over the course of a two-year period. While a preliminary psychometric analysis was conducted on the instrument in 2019 using pilot test data from a single school in Ontario [6], instrument validation is an ongoing, iterative process [7]. Therefore, the purpose of this study was to collect further evidence of validity (specifically, internal structure and relations to other variables) using a larger, more varied sample, representing university students from across Canada.

Methods

Study design

The data analyzed here was collected during the study, “Multi-site Release of the Post-Secondary Student Stressors Index (PSSI)”, conducted during the 2020–2021 academic year. Cross-sectional data were collected at three time points via an online survey from students (n = 12,577) attending fifteen Canadian universities [8]. Participating institutions represented all but one Canadian province. The survey, distributed through Qualtrics, included questions about demographic characteristics, sources of student stress, mental health and mental illness, and stress specific to COVID-19. Participants were provided with a letter of information and indicated their consent by selecting “I have read and understood the Letter of Information and agree to participate in this study” before being granted access to the survey questions. The sampling method and sample size used was at the discretion of each participating institution. Further detail related to study design can be found elsewhere [8].

Measures

Demographics

Several demographic variables were assessed, including: gender (male, female, non-binary), age in years, level of study (undergraduate, graduate, or professional program), marital status, residence during the academic year, student status (full-time/part-time/other), first-generation status (yes/no), international student status (yes/no), self-reported grade point average (GPA), and region of university (Eastern/Western/Central Canada).

Post-secondary student stressors index

The Post-Secondary Student Stressors Index (PSSI) is a 46-item instrument. For each item, respondents are asked to indicate the severity of stress experienced and the frequency with which they experienced it. Responses are provided on an adjectival scale from 1 (‘not stressful’ and ‘rarely’) to 4 (‘extremely stressful’ and ‘almost always’), with higher ratings indicating a greater level of stress resulting from an item. An additional option to indicate “not applicable” (a response of 0) was also available in the event that a stressor didn’t happen or was otherwise not applicable. The tool evaluates stressors across five domains, including: academics, learning environment, campus culture, interpersonal, and personal. For the purposes of the subsequent analyses only severity ratings were used.

Mental health measures

The Perceived Stress Scale (PSS10) is a brief, 10-item scale designed to measure general stress [9]. Participants are asked to indicate the frequency with which they have had certain thoughts or feelings on an adjectival scale from 0 (‘never’) to 4 (‘very often’). Positively worded items on the scale are reverse coded and ratings then summed, with higher scores indicating more perceived stress. The Kessler Psychological Distress Scale (K10) is a 10-item scale designed to measure general psychological distress [10]. Participants are asked to indicate the frequency with which they have had certain thoughts or feelings on an adjectival scale from 1 (‘none of the time’) to 5 (‘all of the time’). Scores are then summed, with higher scores reflecting greater distress and a higher likelihood of experiencing a mental disorder. Both the PSS10 and K10 have demonstrated consistent psychometric properties in a number of samples and settings and have previously been used among post-secondary populations [11–13].

Analysis

The statistical tests we used to collect evidence for validity reflect those appropriate for an index, as opposed to a scale. While both types of multi-item instruments are used to evaluate latent constructs (i.e., variables that cannot be directly observed), items in a scale are referred to as “effect indicators”, while those in an index are referred to as “causal indicators” [7]. With the latter, changes in the items (stressors) determine changes in the value of the latent variable (student stress). The goal of an index is not unidimensionality and internal consistency. On the contrary, there is no expectation for items within an index to correlate with one another, although they might [14, 15]. Instead, index construction places emphasis on considering multicollinearity among indicators and assessing theoretical relationships between latent constructs and predictor variables [16]. Most importantly, while the goal in scale development is often to use as few items as possible to measure the latent construct in the interest of brevity, the opposite is true of index development. Indices often include a large number of items as the goal is to cover the entire scope of the latent variable, which may be quite broad. In general, items in an index are retained as long as they have a distinct influence on the latent construct, as removing an indicator in an index may result in omitting a part of the construct itself [16, 17].

Therefore, to further evaluate the internal structure of the PSSI, we estimated a multiple-indicator, multiple-cause (MIMIC) model, a special case of structural equation modelling (SEM) and an extension of Confirmatory Factor Analysis (CFA) [18]. A MIMIC model utilizes both a psychometric (i.e., measurement) approach as well as a structural approach, which allows for the researcher to estimate relationships among latent constructs (unobserved variables) and contextualize them using predictor variables (usually demographics assumed to have no error, such as age or gender) [18]. We hypothesized that the model would demonstrate that the internal structure of the PSSI was consistent with an index and demonstrate acceptable goodness-of-fit statistics. To assess multicollinearity among indicators, we examined the variance inflation factor (VIF) using a cut-off threshold of 10 [19].

Next, we explored the relationships between the latent variables on the PSSI and other “like” constructs to assess relations to other variables [20]. This method of index validation requires that these constructs are measured using effect indicators and that a theoretical relationship can be presumed to exist between the constructs [17]. We hypothesized that higher scores on the PSSI latent variables (indicating higher student-specific stress) would predict higher scores on both the PSS10 and K10 (higher general stress and psychological distress, respectively) resulting in positive, significant estimates. To test this hypothesis, we specified a second MIMIC model including PSS10 and K10 scores as predicted variables. Next, we calculated nonparametric Spearman’s rho (rs) correlation coefficients to examine the relationships between the PSSI, PSS10, and K10 across a variety of student subgroups, expecting to see positive, moderately significant relationships across all groups.

All tests were conducted using a complete case analysis approach to missing data; any student who had complete information for the variables relevant to an individual test was included in the analysis. All analyses were completed using R, Version 3.4.1. This research received ethics clearance from Queen’s University’s Health Sciences and Affiliated Teaching Hospitals Research Ethics Board.

Results

Sample

Descriptive statistics for the demographic characteristics of the sample are reported in Table 1. Most participants were female (71.6%), single (85.6%), lived off campus with family (54.0%), self-reported their GPA to be in the A range (60.3%), and studied full-time (90.6%) at the undergraduate level (75.0%). Most participants were aged 20 years or younger (41.4%), with an overall average age of 23 (SD = 6.9). Approximately 12% of the sample were international students, and over one quarter of respondents (26%) were the first in their families to attend university.

Table 1. Demographic characteristics of sample (n = 12 577).

Variable n %
Gender
    Female 9007 71.6
    Male 3195 25.4
    Non-binary 238 1.9
    Prefer not to answer 136 1.1
Age
    20 years and under 5205 41.4
    21–24 years 3949 31.4
    25–29 years 1724 13.7
    30 years and over 1683 13.4
Level of Study
    Undergraduate 9437 75.0
    Graduate 2367 18.8
    Professional Program 466 3.7
    Other 260 2.1
    Prefer not to answer 47 0.4
Marital Status
    Single1 10768 85.6
    Married or common-law 1560 12.4
    Separated, Divorced, or Widowed 112 0.9
    Prefer not to answer 137 1.1
Residence During Academic Year
    On campus residence/other 965 7.6
    Off campus with roommates 3346 26.6
    Off campus alone 1267 10.1
    Off campus with family 6794 54.0
    Prefer not to answer 211 1.7
Student Status
    Full-time 11390 90.6
    Part-time 1064 8.5
    Other 92 0.7
    Prefer not to answer 31 0.2
First Generation Student
    No 9247 73.5
    Yes 3263 25.9
    Prefer not to answer 67 0.5
International Student
    No 10962 87.2
    Yes 1590 12.6
    Prefer not to answer 25 0.2
GPA
    A 7580 60.3
    B 3608 28.7
    C 779 6.2
    D/F 61 0.5
    Prefer not to answer 542 4.3
Region
    Atlantic Canada 4364 35.6
    Western Canada 5441 44.4
    Central Canada 2445 20.0

(1) “Single” includes respondents who indicated they were ‘single and not dating’, ‘single and dating’, and in a relationship (not married)’

Internal structure evidence

All 46 items on the PSSI were first correlated with the PSS10 in line with recommendations to explore correlations between index indicators and a separate variable that “summarizes the essences of the construct that the index purports to measure” [16, 17]. The PSS10 is a validated measure of general perceived stress, thus fulfilling this requirement. We assessed the potential for multicollinearity by examining the variance inflation factor (VIF) for each indicator. All VIF values were well below the cut-off (<5).

As previously outlined, the PSSI was conceptualized as being an index composed of five domains (or latent constructs) of student stress: academics, learning environment, campus culture, interpersonal, and personal stressors. As such, we specified these five latent variables in our MIMIC model and included manifest variables age and gender (where 1 = female and 0 = male) as predictors for our structural model. Descriptive statistics for indicators and covariates are shown in Table 2. We decided to run two models. In the first model, we excluded observations where a participant indicated that a stressor was “not applicable” or did not happen. This resulted in a model with 516 total observations. In the second model, we included those observations, resulting in a model with 11,965 total observations. Results of both models are displayed in Table 3.

Table 2. Descriptive statistics for indicators and covariates.

Female (n = 9007) Male (n = 3195)
Mean SD % NA Mean SD % NA
Covariates
Age 23.4 6.8 -- 23.9 7.2 --
PSS10 23.2 6.1 -- 20.6 6.6 --
K10 29.3 9.4 -- 26.3 9.4 --
Indicators
Academic
    Preparing for exams 2.8 0.8 8.0 2.5 0.8 5.4
    Writing exams 2.9 0.8 8.4 2.5 0.9 5.9
    Writing multiple exams around the same time 3.5 0.7 20.5 3.2 0.8 18.9
    Exams worth more than 50% of course grade 3.4 0.7 17.8 3.0 0.9 13.6
    Heavily weighted assignments 2.7 0.8 3.5 2.4 0.9 3.1
    Having multiple assignments due around the same time 3.0 0.8 3.3 2.8 0.9 3.3
    Managing my academic workload 2.6 0.8 0.5 2.4 0.9 0.6
    Receiving a bad grade 3.2 0.8 5.8 2.9 0.9 5.5
    Managing a high GPA 2.9 0.9 2.9 2.5 1.0 4.0
    Working on my thesis 2.8 0.9 67.0 2.6 0.9 62.5
    Performing well at my professional placement (i.e., practicum, clerkship) 2.6 0.9 57.7 2.3 0.9 56.3
Learning Environment
    Poor communication from professor 2.9 0.8 5.9 2.7 0.9 5.9
    Unclear expectations from professor 3.0 0.8 4.3 2.8 0.9 5.1
    Lack of guidance from professor 2.8 0.8 5.4 2.6 0.9 5.8
    Meeting with my professor 2.0 1.0 13.5 1.7 0.9 12.5
    Meeting my thesis/placement supervisor’s expectations 2.5 0.9 61.5 2.3 0.9 59.6
    Lack of mentoring from my thesis/placement supervisor 2.6 1.0 66.9 2.4 1.0 63.7
Campus Culture
    Adjusting to the post-secondary lifestyle 2.1 0.9 6.9 1.9 0.9 6.9
    Adjusting to my program 2.1 0.9 4.2 1.9 0.8 4.3
    Academic competition among my peers 2.1 1.0 7.5 1.8 0.9 9.4
    Feeling like I’m not working hard enough 2.9 0.9 2.9 2.7 0.9 3.6
    Feeling like my peers are smarter than I am 2.6 1.0 4.3 2.2 1.1 6.9
    Pressure to succeed 3.0 0.9 1.1 2.8 1.0 2.1
    Discrimination on campus 2.0 1.0 59.5 1.9 1.0 61.8
    Sexual harassment on campus 2.3 1.0 62.2 1.8 1.0 73.5
Interpersonal
    Making new friends 2.1 1.0 7.9 1.9 1.0 8.8
    Maintaining friendships 2.0 0.9 3.8 1.8 0.9 5.2
    Networking with the "right" people 2.2 0.9 9.4 2.1 0.9 10.1
    Feeling pressured to socialize 2.2 1.0 10.0 1.9 1.0 12.6
    Balancing a social life with academics 2.5 0.9 2.9 2.3 1.0 4.2
    Comparing myself to others 2.7 1.0 4.1 2.3 1.0 7.5
    Comparing my life to others’ on social media 2.3 1.0 11.4 1.9 1.0 24.9
    Meeting other peoples’ expectations of me 2.6 1.0 4.0 2.3 1.0 8.7
    Meeting my own expectations 3.1 0.9 0.5 2.8 1.0 1.4
Personal
    Making sure that I get enough sleep 2.3 1.0 0.9 2.1 1.0 1.8
    Making sure that I get enough exercise 2.4 0.9 2.6 2.2 0.9 2.8
    Making sure that I eat healthy 2.3 0.9 1.2 2.0 0.9 2.3
    Having to prepare meals for myself 2.0 1.0 5.8 1.8 0.9 8.9
    Balancing working at my job with my academics 2.7 0.9 30.8 2.5 1.0 38.5
    Balancing my extracurriculars with academics 2.2 0.9 25.4 2.1 0.9 24.8
    Feeling guilty about taking time for my hobbies/interests 2.7 1.0 4.7 2.4 1.0 7.5
    Having to take student loans 2.8 1.1 50.7 2.5 1.1 52.1
    Worrying about paying off debt 2.9 1.0 40.3 2.6 1.1 43.0
    Worrying about getting a job after graduating 2.9 1.0 7.9 2.7 1.1 9.5
    Worrying about getting into a new program after graduating 2.8 1.1 29.2 2.4 1.1 32.5
    Worrying about reaching major "life events" (i.e., buying a house, marriage) 2.8 1.0 8.4 2.5 1.1 12.0

(1) PSSI responses range from 1 to 4 where higher scores are equivalent to higher severity of stress.

(2) # NA column indicates the proportion of participants indicating that the stressor was “not applicable” or did not happen. These responses were removed from mean calculations.

Table 3. Multiple cause, multiple indicator (MIMIC) model estimates.

Parameter Indicators Latent Variable Estimates
Model 1 Model 2
n = 516 n = 11 965
Factor Loading Preparing for exams Academic 0.717 0.793
Writing exams 0.728 0.787
Writing multiple exams around the same time 0.702 0.678
Exams worth more than 50% of course grade 0.693 0.710
Heavily weighted assignments 0.710 0.576
Having multiple assignments due around the same time 0.733 0.577
Managing my academic workload 0.746 0.451
Receiving a bad grade 0.691 0.618
Managing a high GPA 0.737 0.624
Working on my thesis 0.585 -0.025
Performing well at my professional placement (i.e., practicum, clerkship) 0.620 0.189
Poor communication from professor Learning Env 0.802 0.824
Unclear expectations from professor 0.830 0.858
Lack of guidance from professor 0.844 0.826
Meeting with my professor 0.587 0.331
Meeting my thesis/placement supervisor’s expectations 0.619 0.090
Lack of mentoring from my thesis/placement supervisor 0.705 0.185
Adjusting to the post-secondary lifestyle Campus Culture 0.769 0.533
Adjusting to my program 0.794 0.537
Academic competition among my peers 0.798 0.644
Feeling like I’m not working hard enough 0.774 0.696
Feeling like my peers are smarter than I am 0.790 0.747
Pressure to succeed 0.784 0.729
Discrimination on campus 0.505 0.305
Sexual harassment on campus 0.489 0.303
Making new friends Interpersonal 0.730 0.559
Maintaining friendships 0.732 0.589
Networking with the "right" people 0.812 0.566
Feeling pressured to socialize 0.766 0.600
Balancing a social life with academics 0.791 0.622
Comparing myself to others 0.810 0.765
Comparing my life to others’ on social media 0.749 0.641
Meeting other peoples’ expectations of me 0.803 0.700
Meeting my own expectations 0.711 0.622
Making sure that I get enough sleep Personal 0.682 0.598
Making sure that I get enough exercise 0.739 0.654
Making sure that I eat healthy 0.715 0.693
Having to prepare meals for myself 0.655 0.551
Balancing working at my job with my academics 0.752 0.380
Balancing my extracurriculars with academics 0.761 0.409
Feeling guilty about taking time for my hobbies/interests 0.744 0.581
Having to take student loans 0.662 0.411
Worrying about paying off debt 0.686 0.433
Worrying about getting a job after graduating 0.692 0.504
Worrying about getting into a new program after graduating 0.681 0.403
Worrying about reaching major "life events" (i.e., buying a house, marriage) 0.714 0.540
Regression Coefficient Age Academic -0.012 ** -0.038 ***
(0.004) (0.001)
Gender Academic 0.334 (0.059) *** 0.229 (0.017) ***
Age Learning Env -0.005 (0.005) -0.018 (0.001) ***
Gender Learning Env 0.247 (0.073) *** 0.184 (0.019) ***
Age Campus Culture -0.011 (0.005) * -0.021 (0.001) ***
Gender Campus Culture 0.310 (0.078) *** 0.232 (0.012) ***
Age Interpersonal -0.012 (0.005) * -0.022 (0.001) ***
Gender Interpersonal 0.213 (0.077) ** 0.296 (0.014) ***
Age Personal -0.007 (0.005) -0.008 (0.001) ***
Gender Personal 0.271 (0.067) *** 0.253 (0.014) ***

(1) Model 1 fit statistics: X2 = 4218, df = 1061, p<0.001, RMSEA = 0.076 (95% CI 0.074, 0.078), SRMR = 0.057, CFI = 0.810, TLI = 0.800.

(2) Model 2 fit statistics: X2 = 92240, df = 1061, p<0.001, RMSEA = 0.085 (95% CI 0.084, 0.085), SRMR = 0.082, CFI = 0.640, TLI = 0.618.

(3) Regression coefficient estimates β (Standard Error). Indicates statistical significance at

* p<0.05, ** p<0.01, *** p<0.001.

In the first model, gender was significantly associated with all latent constructs, while age was significantly associated with all but the “learning environment” and “personal”. All items demonstrated strong factor loadings (>0.5). The model was statistically significant (X2 = 4218, df = 1061, p<0.001) and demonstrated good overall fit. The root mean square error of approximation (RMSEA) and standardized root mean square residual (SRMR) were both <1, and both the Comparative Fit Index (CFI) and Tucker-Lewis Index (TLI) were only just below the desired cut-off of ≥0.90 at 0.81 and 0.80, respectively. In contrast, the second model revealed several low (and in one case, negative) factor loadings and weaker overall fit statistics. The lower factor loadings were observed on items where a much larger proportion of respondents indicated that the stressor was “not applicable” or did not happen to them relative to the other items on the index. In this model, gender and age were significantly associated with all latent constructs, though this is to be expected given the large sample size. Similarly, though the model itself was found to be significant, the chi-square statistic is sensitive to sample size, and thus not informative. Ultimately, results suggest that the removal of responses of “not applicable” or “did not happen” results in a stronger overall model.

Relations to other variables

To assess evidence for relations to other variables, we first specified a third MIMIC model including PSS10 and K10 scores as predicted variables (Table 4). Estimation of the model resulted in a good overall fit and, as expected, the path between the PSSI latent variables and selected indicators were significant and above 0, consistent with our hypothesis.

Table 4. MIMIC model estimates exploring relations to other variables (n = 516).

Indicators Latent Variable Estimate (SE) p
PSS10 Academic 0.019 (0.006) 0.001
K10 0.028 (0.003) <0.001
PSS10 Learning Env 0.016 (0.008) 0.041
K10 0.028 (0.004) <0.001
PSS10 Campus Culture 0.021 (0.008) 0.007
K10 0.039 (0.004) <0.001
PSS10 Interpersonal 0.026 (0.007) <0.001
K10 0.037 (0.004) <0.001
PSS10 Personal 0.026 (0.006) <0.001
K10 0.033 (0.004) <0.001

(1) Model fit statistics: RMSEA = 0.076 (95% CI 0.074, 0.078), SRMR = 0.057, CFI = 0.811, TLI = 0.799, X2 = 4348, df = 1061, p<0.001.

(2) SE = Standard error

Next, we assessed the correlations between the PSSI and like instruments, using a non-parametric Spearman’s rho as the data were not normally distributed. Given that it would not be appropriate to sum participants’ responses on the PSSI, we created a “count” variable. The goal of the PSSI is not to derive a comprehensive severity “score” based on the stressors experienced, but rather to identify sources of stress. Logically, we would expect that those who experienced a greater number of stressors would in turn experience a higher level of overall stress and/or psychological distress, as measured by the PSS10 and K10. Thus, to develop the PSSI count variable, we dichotomized responses such that any response indicating stress in response to a stressor was coded as 1, and responses indicating that a stressor was “not applicable”, did not happen, or was not stressful were coded as 0. Counts were then summed to derive a count. Essentially, this applies an equal weight of 1 to each stressor, a common method employed when working with health status indices [7]. To assess these relationships across different subgroups of students, we repeated the analysis across several split samples. Results are displayed in Table 5. As hypothesized, the analyses revealed moderate, positive correlations across all groups. All correlations were significant at p<0.001.

Table 5. Relations to other variables (Spearman’s rho correlations (rs)) by group.

Group PSSI and K10 PSSI and PSS10
r s p 95% CI r s p 95% CI
Total Sample 0.47 <0.001 0.45, 0.48 0.44 <0.001 0.43, 0.46
Sex
    Female 0.44 <0.001 0.42, 0.45 0.40 <0.001 0.38, 0.42
    Male 0.51 <0.001 0.48, 0.54 0.51 <0.001 0.48, 0.53
Level of Study
    Undergraduate 0.46 <0.001 0.44, 0.48 0.44 <0.001 0.42, 0.45
    Graduate 0.46 <0.001 0.42, 0.49 0.45 <0.001 0.42, 0.48
Course Load
    Full-time 0.46 <0.001 0.44, 0.47 0.44 <0.001 0.42, 0.45
    Part-time 0.52 <0.001 0.47, 0.57 0.49 <0.001 0.44, 0.53
Status
    Domestic 0.46 <0.001 0.44, 0.48 0.45 <0.001 0.43, 0.46
    International 0.51 <0.001 0.47, 0.55 0.45 <0.001 0.41, 0.49

Discussion

This psychometric analysis followed the guidelines laid out by Diamantopoulos and Winklhofer regarding validation processes appropriate for an instrument developed during a formative measurement perspective (i.e., an index rather than a scale) [16, 17]. Notably, no reliability analyses nor dimensionality tests, such as exploratory factor analysis, were performed on the PSSI as unidimensionality and internal consistency are not relevant when evaluating the validity of an index [14]. The results of the analyses presented here echo the preliminary analyses previously published [6] and provide further support for the validity of the Post-Secondary Student Stressors Index (PSSI). Results demonstrate its utility among a large sample of students who varied by gender, age, level of study, course load (i.e., part-time vs. full-time), and more.

Unlike other tools that purport to evaluate the sources of student stress, the PSSI was co-developed with students, and has now been validated using a large sample of students attending university across Canada. The PSSI is a valuable tool for universities that are interested in identifying the presence and distribution of student stressors on their campuses. A more in-depth understanding of the sources of student stress, as well as their associated severity and frequency is an important step towards improved tailoring of upstream campus mental health and wellbeing services. Although downstream campus services (i.e., psychotherapy, pharmacotherapy) continue to be overburdened, few post-secondary institutions have successfully taken a comprehensive or “whole campus approach” including both upstream and downstream mental health services, as is recommended by broad frameworks like the Okanagan Charter. Post-secondary institutions are increasingly encouraged to incorporate both physical and mental health into institutional culture and policies to promote an environment that enhances health and well-being, instead of focusing on downstream treatment only [21]. By improving understanding of the sources of student stress and areas most in need of support through the use of tools like the PSSI, institutions may be able to improve the effectiveness of upstream services, thereby contributing toward alleviating the bottleneck currently observed at the downstream service level.

Limitations

There are some limitations to this research. While the results demonstrate additional evidence for the validity of the tool among students attending schools across a variety of regions in Canada, the sample did not include participants from the province of Quebec. Additionally, the French version of the PSSI has not yet been formally tested. As a result, the findings presented here cannot be generalized to the francophone university student population in Canada. Secondly, the PSSI, though intended for use cross various types of post-secondary institutions, has thus far only been validated for use among university student populations. Therefore, caution should be used when applying this tool among samples of post-secondary students attending colleges, institutes, etc. Finally, the data used for this study were collected during the 2020–2021 academic year, which was the first full academic year that took place during the global COVID-19 pandemic. As a result, it is likely that stress levels (both general and student-specific) were somewhat higher than we might usually expect. However, this would only be expected to impact the descriptive analyses, and not the psychometric analyses.

Conclusion

The results of this research provide further evidence for the validity of the PSSI among varied university student populations in Canada and addresses several of the limitations identified in earlier preliminary psychometric work on the instrument. Future research will continue to explore the utility of the PSSI in identifying distributions of stressors among unique subgroups of interest (i.e., international students, first-generation students, etc.), as well as the continued validation of the instrument in different contexts (i.e., French-speaking students, college populations). Finally, the development of a shortened version of the PSSI to improve its accessibility and utility in terms of its ability to be added on to existing survey instruments will also be explored.

Acknowledgments

BL would like to acknowledge and thank all the students who participated in this study, as well as each of the co-investigators at participating institutions who offered their time and commitment to this project during a challenging academic year.

Data Availability

There are ethical restrictions to sharing this data, put in place by the 15 ethics review board(s) from which we received clearance. A stipulation of our ethics approvals across all sites was that the students’ data would not be shared without an additional layer of ethics approval. All secondary analyses require a subsequent ethics request to the Queen’s University’s Health Sciences and Affiliated Teaching Hospitals Research Ethics Board (HSREB) as the primary ethics body that approved this study. Requests regarding access to the data used in this study can be directed to the HSREB (hsreb@queensu.ca, 613-533-6000 ext. 78223), who will forward these requests to the data custodian (Dr. Brooke Linden, brooke.linden@queensu.ca). We are more than happy to share the data with other investigators, and encourage collaborative analyses.

Funding Statement

The authors received no specific funding for this work.

References

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Decision Letter 0

Lorena Verduci

2 Aug 2022

PONE-D-22-06413

Further evidence in support of the validity of the Post-Secondary Student Stressors Index using a nationwide, cross-sectional sample of Canadian university students

PLOS ONE

Dear Dr. Linden,

Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process.

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The manuscript has been evaluated by one reviewer, and his comments are available below.

The reviewer has raised a number of concerns. He requests improvements to the reporting of methodological aspects of the study, for example, regarding the characteristics of the institutions and definition of post-secondary education. The reviewer also requests revision to discussion.

Could you please carefully revise the manuscript to address all comments raised?

Please submit your revised manuscript by Sep 16 2022 11:59PM. If you will need more time than this to complete your revisions, please reply to this message or contact the journal office at plosone@plos.org. When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file.

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Staff Editor

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Reviewers' comments:

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Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

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3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

**********

5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: This manuscript is a continuation of previous studies that provides additional evidence to support the use of an indicator on stressors in students in Canada. With this, it seeks to identify the main sources of stressors and improve mental health services.

The creation of the instrument and its validation fills a gap well exposed by the researchers related to the fact that there is no such tool with the participatory approach that is proposed.

Major comments

1. Do the participating institutions have the same characteristics? I am referring to whether there may be different sources of stress depending on the profile of the participating institution. Perhaps it would help to mention that the type of institutions was comparable if they were.

2. Is there a difference between post-secondary education and higher education? I understand that it includes not only universities, but also other types of educational institutions. I recommend clarifying what you mean by post-secondary education.

3. According to the domains of stressors and their respective items, (although the development of the instrument should be in another publication) could you describe how these were chosen and how was this a participatory process?

4. I recommend getting more out of the results in the discussion. An important topic that could be included in the discussion is that when identifying the sources of stress, it comes to light that most of them have nothing to do with the quality and quantity of mental health services in institutions. Rather, the sources of stress come from, for example, scheduling, organization and evaluation methods, communication and relationship between teachers and students, or even sexual harassment on campus. How do the authors think these sources of stress can be addressed on campus? can they be addressed? is there an example of that?

Minor comments

References. Data presentations, spelling, grammar, reagent word.

1. I recommend changing the post-secondary keyword since it's in the title.

2. There is an error in the results since it says that the ages range from 16 to 20 years and in the table it is up to 30.

**********

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Reviewer #1: No

**********

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PLoS One. 2022 Dec 15;17(12):e0278897. doi: 10.1371/journal.pone.0278897.r002

Author response to Decision Letter 0


20 Sep 2022

Response to Reviewers

Manuscript #PONE-D-22-06413: “Further evidence in support of the validity of the Post-Secondary Student Stressors Index using a nationwide, cross-sectional sample of Canadian university students”

Thank you to the reviewers for the opportunity to revise this manuscript. Please find below point-by-point responses to your inquiries, comments, and suggestions.

Reviewer #1:

This manuscript is a continuation of previous studies that provides additional evidence to support the use of an indicator on stressors in students in Canada. With this, it seeks to identify the main sources of stressors and improve mental health services. The creation of the instrument and its validation fills a gap well exposed by the researchers related to the fact that there is no such tool with the participatory approach that is proposed.

Comment 1: Do the participating institutions have the same characteristics? I am referring to whether there may be different sources of stress depending on the profile of the participating institution. Perhaps it would help to mention that the type of institutions was comparable if they were.

Response: Thank you for this question. In fact, this was one of the questions we sought to answer through this cross-Canada, multi-site study, and indeed something that we are investigating in additional analyses. For example, we were interested in learning whether patterns of stress would appear to be different based on region in Canada, size of campus, and nature of campus (with respect to them being “commuter” campuses, where students typically live off campus and drive in to attend school vs. non-commuter campuses, where the vast majority of students live on or very near campus). These are all research questions that are being answered in additional sub-studies of this data, as they are beyond the scope of this particular paper.

For the purposes of this paper, we sought to investigate whether the psychometric properties of the index would remain stable and similar to what we observed in our pilot study at a single, Eastern Ontario university. In fact, we note that a major goal of the study was to collect further evidence of validity using a larger, more varied sample (line 66-68) – therefore the strong psychometrics our analyses revealed using this sample given the hypothesized variation in institutions’ characteristics lends additional strength to our findings.

Comment 2: Is there a difference between post-secondary education and higher education? I understand that it includes not only universities, but also other types of educational institutions. I recommend clarifying what you mean by post-secondary education.

Response: No, “post-secondary education” and “higher education” are synonymous. The PSSI was developed and intended to be used across different types of higher education institutions, including universities and colleges (hence “post-secondary stressors index”, and not “university stressors index”). However, the reviewer has likely noted that the majority of the participating institutions in this sample were universities (the one exception being Aurora College). Admittedly, this is a limitation of the tool as it has not yet been widely tested among college populations (this is in the works).

In response to this comment, we have now reviewed all instances throughout the manuscript where we have referred to “post-secondary” and adjusted this to “university” as appropriate (note that the word university is used when we refer to our sample or the applicability of the PSSI, but when referencing general mental health statistics for post-secondary student populations, for example, the word post-secondary is used as these stats apply to both university and college populations. We have also added a statement in our limitations section regarding the lack of testing to date among college populations potentially limiting the generalizability of the PSSI in that context (lines 283-286).

Comment 3: According to the domains of stressors and their respective items, (although the development of the instrument should be in another publication) could you describe how these were chosen and how was this a participatory process?

Response: The reviewer is correct that details regarding the development of the PSSI have been published elsewhere (please see Linden & Stuart 2019 [doi: 10.1186/s12889-019-7472-z] and Linden, Boyes & Stuart 2020 [doi: 10.1080/07448481.2020.1754222]). Briefly, the PSSI was developed through detailed consultation with students, including the selection and naming of the domains. Naming of the domains was decided over the course of multiple focus group interviews with diverse groups of students.

Comment 4: I recommend getting more out of the results in the discussion. An important topic that could be included in the discussion is that when identifying the sources of stress, it comes to light that most of them have nothing to do with the quality and quantity of mental health services in institutions. Rather, the sources of stress come from, for example, scheduling, organization and evaluation methods, communication and relationship between teachers and students, or even sexual harassment on campus. How do the authors think these sources of stress can be addressed on campus? can they be addressed? is there an example of that?

Response: With respect to this component of the author’s statement: “…when identifying the sources of stress, it comes to light that most of them have nothing to do with the quality and quantity of mental health services in institutions”, we are unable to comment as we did not do any analytic work that might provide support for a relationship (or lack of relationship) between he quality/quantity of mental health services provided on campuses and the stressors students are experiencing. While we appreciate the reviewer’s recommendation to add more discussion around the specific items that students identified as impactful sources of stress, we feel that would be beyond the scope of the present paper, which aims only to provide a concise assessment of the instrument’s psychometric properties. In fact, we have recently published an alternative paper that goes into detail regarding this exact question, exploring the stressors identified by students as impactful across each of the domains, as well as looking at whether trends appeared over time. We invite the reviewer to refer to Linden, Stuart, and Ecclestone 2022 (doi: 10.1177/07067437221111365).

Comment 5: I recommend changing the post-secondary keyword since it's in the title.

Response: This keyword has now been changed to “university”.

Comment 6: There is an error in the results since it says that the ages range from 16 to 20 years and in the table it is up to 30.

Response: If the reviewer is referring to lines 167-168 in the “Sample” subsection under results, we state that the majority of respondents fell between the ages of 16 and 20, not all of the respondents (41.4% of our sample was under the age of 21, while the other age categories account for considerable smaller proportions of the sample). For clarity we have adjusted this sentence to read “Most participants were aged 20 years or younger…” to better align with the age categories as displayed in Table 1. Please note that in reviewing this paragraph, we did notice some inconsistencies in the proportions reported in text - these have all now been fixed to align with the numbers in the table (lines 164-169).

Attachment

Submitted filename: Response to Reviewers.docx

Decision Letter 1

Supat Chupradit

14 Nov 2022

PONE-D-22-06413R1Further evidence in support of the validity of the Post-Secondary Student Stressors Index using a nationwide, cross-sectional sample of Canadian university studentsPLOS ONE

Dear Dr. Linden,

Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process.

Please submit your revised manuscript by Dec 29 2022 11:59PM. If you will need more time than this to complete your revisions, please reply to this message or contact the journal office at plosone@plos.org. When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file.

Please include the following items when submitting your revised manuscript:

  • A rebuttal letter that responds to each point raised by the academic editor and reviewer(s). You should upload this letter as a separate file labeled 'Response to Reviewers'.

  • A marked-up copy of your manuscript that highlights changes made to the original version. You should upload this as a separate file labeled 'Revised Manuscript with Track Changes'.

  • An unmarked version of your revised paper without tracked changes. You should upload this as a separate file labeled 'Manuscript'.

If you would like to make changes to your financial disclosure, please include your updated statement in your cover letter. Guidelines for resubmitting your figure files are available below the reviewer comments at the end of this letter.

If applicable, we recommend that you deposit your laboratory protocols in protocols.io to enhance the reproducibility of your results. Protocols.io assigns your protocol its own identifier (DOI) so that it can be cited independently in the future. For instructions see: https://journals.plos.org/plosone/s/submission-guidelines#loc-laboratory-protocols. Additionally, PLOS ONE offers an option for publishing peer-reviewed Lab Protocol articles, which describe protocols hosted on protocols.io. Read more information on sharing protocols at https://plos.org/protocols?utm_medium=editorial-email&utm_source=authorletters&utm_campaign=protocols.

We look forward to receiving your revised manuscript.

Kind regards,

Supat Chupradit, Ph.D., M.Ed., B.Sc.(OT), B.P.A., B.Ed., B.A.

Academic Editor

PLOS ONE

Journal Requirements:

Please review your reference list to ensure that it is complete and correct. If you have cited papers that have been retracted, please include the rationale for doing so in the manuscript text, or remove these references and replace them with relevant current references. Any changes to the reference list should be mentioned in the rebuttal letter that accompanies your revised manuscript. If you need to cite a retracted article, indicate the article’s retracted status in the References list and also include a citation and full reference for the retraction notice.

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #1: All comments have been addressed

Reviewer #2: All comments have been addressed

Reviewer #3: All comments have been addressed

Reviewer #4: All comments have been addressed

**********

2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

Reviewer #3: Yes

Reviewer #4: Yes

**********

3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

Reviewer #3: Yes

Reviewer #4: Yes

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

Reviewer #3: Yes

Reviewer #4: Yes

**********

5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

Reviewer #3: Yes

Reviewer #4: Yes

**********

6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: I have reviewed the responses given by the authors to my comments. I am satisfied with the answers and recommend the publication of this manuscript.

Reviewer #2: Overall, this paper is quite interesting and has been revised based on the feedback of a previous reviewer. However, there are some areas that need improvement. The Abstract should be written in one paragraph only. The demographic data of the samples should be mentioned in the Research method, not the Research results.

Reviewer #3: Further evidence in support of the validity of the Post-Secondary Student Stressors Index using a nationwide, cross-sectional sample of Canadian university students. This manuscript revision, I'm accept all comments that author response. Accept

Reviewer #4: Thank you for considering my comments and suggestions. I am satisfied with the responses.

All the best for your article.

**********

7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: Yes: Mónica Suárez-Reyes

Reviewer #2: Yes: Kittisak JERMSITTIPARSERT

Reviewer #3: No

Reviewer #4: No

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PLoS One. 2022 Dec 15;17(12):e0278897. doi: 10.1371/journal.pone.0278897.r004

Author response to Decision Letter 1


14 Nov 2022

Response to Reviewers

Manuscript #PONE-D-22-06413: “Further evidence in support of the validity of the Post-Secondary Student Stressors Index using a nationwide, cross-sectional sample of Canadian university students”

Thank you to the reviewers for the opportunity to revise this manuscript. Please find below point-by-point responses to your inquiries, comments, and suggestions.

Journal Requirements:

Comment 1: Please review your reference list to ensure that it is complete and correct. If you have cited papers that have been retracted, please include the rationale for doing so in the manuscript text, or remove these references and replace them with relevant current references. Any changes to the reference list should be mentioned in the rebuttal letter that accompanies your revised manuscript. If you need to cite a retracted article, indicate the article’s retracted status in the References list and also include a citation and full reference for the retraction notice.

Response: The reference list (and in-text citations) have now been reviewed and are correct.

Reviewer Comments:

Comment 1: The Abstract should be written in one paragraph only.

Response: We have now shortened the abstract to be the length of a single paragraph.

Comment 2: The demographic data of the samples should be mentioned in the Research method, not the Research results.

Response: We have elected not to make this requested change as we feel it is more appropriate to have the demographic statistical results reported in the Results section with all other results.

Attachment

Submitted filename: Response to Reviewers.docx

Decision Letter 2

Supat Chupradit

25 Nov 2022

Further evidence in support of the validity of the Post-Secondary Student Stressors Index using a nationwide, cross-sectional sample of Canadian university students

PONE-D-22-06413R2

Dear Dr. Linden,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

An invoice for payment will follow shortly after the formal acceptance. To ensure an efficient process, please log into Editorial Manager at http://www.editorialmanager.com/pone/, click the 'Update My Information' link at the top of the page, and double check that your user information is up-to-date. If you have any billing related questions, please contact our Author Billing department directly at authorbilling@plos.org.

If your institution or institutions have a press office, please notify them about your upcoming paper to help maximize its impact. If they’ll be preparing press materials, please inform our press team as soon as possible -- no later than 48 hours after receiving the formal acceptance. Your manuscript will remain under strict press embargo until 2 pm Eastern Time on the date of publication. For more information, please contact onepress@plos.org.

Kind regards,

Supat Chupradit, Ph.D., M.Ed., B.Sc.(OT), B.P.A., B.Ed., B.A.

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #1: All comments have been addressed

Reviewer #3: All comments have been addressed

Reviewer #4: All comments have been addressed

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2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #3: Yes

Reviewer #4: Yes

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3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #3: Yes

Reviewer #4: Yes

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4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #3: Yes

Reviewer #4: Yes

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5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #3: Yes

Reviewer #4: Yes

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6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: The authors have responded to my comments satisfactorily. The article is interesting and provides relevant information on the study of health promotion in the university context. I recommend accepting the article.

Reviewer #3: Further evidence in support of the validity of the Post-Secondary Student Stressors Index using a nationwide, cross-sectional sample of Canadian university students. Overall improve by reviewer's comments. accept.

Reviewer #4: Further evidence in support of the validity of the Post-Secondary Student Stressors Index using a nationwide, cross-sectional sample of Canadian university students. I approve this manuscript that revise version.

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Reviewer #1: Yes: Mónica Suárez-Reyes

Reviewer #3: No

Reviewer #4: No

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Acceptance letter

Supat Chupradit

7 Dec 2022

PONE-D-22-06413R2

Further evidence in support of the validity of the Post-Secondary Student Stressors Index using a nationwide, cross-sectional sample of Canadian university students

Dear Dr. Linden:

I'm pleased to inform you that your manuscript has been deemed suitable for publication in PLOS ONE. Congratulations! Your manuscript is now with our production department.

If your institution or institutions have a press office, please let them know about your upcoming paper now to help maximize its impact. If they'll be preparing press materials, please inform our press team within the next 48 hours. Your manuscript will remain under strict press embargo until 2 pm Eastern Time on the date of publication. For more information please contact onepress@plos.org.

If we can help with anything else, please email us at plosone@plos.org.

Thank you for submitting your work to PLOS ONE and supporting open access.

Kind regards,

PLOS ONE Editorial Office Staff

on behalf of

Assistant Professor Supat Chupradit

Academic Editor

PLOS ONE

Associated Data

    This section collects any data citations, data availability statements, or supplementary materials included in this article.

    Supplementary Materials

    Attachment

    Submitted filename: Response to Reviewers.docx

    Attachment

    Submitted filename: Response to Reviewers.docx

    Data Availability Statement

    There are ethical restrictions to sharing this data, put in place by the 15 ethics review board(s) from which we received clearance. A stipulation of our ethics approvals across all sites was that the students’ data would not be shared without an additional layer of ethics approval. All secondary analyses require a subsequent ethics request to the Queen’s University’s Health Sciences and Affiliated Teaching Hospitals Research Ethics Board (HSREB) as the primary ethics body that approved this study. Requests regarding access to the data used in this study can be directed to the HSREB (hsreb@queensu.ca, 613-533-6000 ext. 78223), who will forward these requests to the data custodian (Dr. Brooke Linden, brooke.linden@queensu.ca). We are more than happy to share the data with other investigators, and encourage collaborative analyses.


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