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. 2022;2450:493–508. doi: 10.1007/978-1-0716-2172-1_26

Random Integration Transgenesis in a Free-Living Regenerative Flatworm Macrostomum lignano.

Jakub Wudarski, Kirill Ustyantsev, Filipa Reinoite, Eugene Berezikov
PMCID: PMC9761508  PMID: 35359325

Abstract

Regeneration-capable flatworms are highly informative research models to study the mechanisms of stem cell regulation, regeneration, and tissue patterning. Transgenesis is a powerful research tool for investigating gene function, but until recently, a transgenesis method was missing in flatworms, hampering their wider adoption in biomedical research. Here we describe a detailed protocol to create stable transgenic lines of the flatworm M. lignano using random integration of DNA constructs through microinjection into single-cell stage embryos.

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