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. 2022;2450:179–194. doi: 10.1007/978-1-0716-2172-1_9

Studying Annelida Regeneration in a Novel Model Organism: The Freshwater Aeolosoma viride.

Chiao-Ping Chen, Sheridan Ke-Wing Fok, Cheng-Yi Chen, Fei-Man Hsu, Yu-Wen Hsieh, Jiun-Hong Chen
PMCID: PMC9761521  PMID: 35359308

Abstract

Aeolosoma viride, a globally distributed freshwater annelid, has a semitransparent appearance with 10 to 12 segments, about 2 to 3 mm in length. It is easy to raise and handle in laboratory conditions. Due to its robust regenerative capacity and applicability of various molecular tools including EdU labeling, whole-mount in situ hybridization (WISH), and RNA interference (RNAi), it rises as a promising model for studying whole-body regeneration.

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References

  1. Bely AE (1999) Decoupling of fission and regeneration capabilities in an asexual oligochaete. Hydrobiologia 406:243–251. https://doi.org/10.1007/978-94-011-4207-6_24 doi: 10.1007/978-94-011-4207-6_24. [DOI]
  2. Özpolat BD, Bely AE (2016) Developmental and molecular biology of annelid regeneration: a comparative review of recent studies. Curr Opin Genet Dev 40:144–153. https://doi.org/10.1016/j.gde.2016.07.010 doi: 10.1016/j.gde.2016.07.010. [DOI] [PubMed]
  3. Falconi R, Gugnali A, Zaccanti F (2015) Quantitative observations on asexual reproduction of Aeolosoma viride (Annelida, Aphanoneura). Invertebr Biol 134:151–161. https://doi.org/10.1111/ivb.12087 doi: 10.1111/ivb.12087. [DOI]
  4. Chen CP, Fok SKW, Hsieh YW et al (2020) General characterization of regeneration in Aeolosoma viride (Annelida, Aeolosomatidae). Invertebr Biol 139:e12277. https://doi.org/10.1111/ivb.12277 doi: 10.1111/ivb.12277. [DOI]
  5. Hessling R, Purschke G (2000) Immunohistochemical (cLSM) and ultrastructural analysis of the central nervous system and sense organs in Aeolosoma hemprichi (Annelida, Aeolosomatidae). Zoomorphology 120:65–78. https://doi.org/10.1007/s004350000022 doi: 10.1007/s004350000022. [DOI]
  6. Zrzavý J, Říha P, Piálek L et al (2009) Phylogeny of Annelida (Lophotrochozoa): total-evidence analysis of morphology and six genes. BMC Evol Biol 9:1–14. https://doi.org/10.1186/1471-2148-9-189 doi: 10.1186/1471-2148-9-189. [DOI] [PMC free article] [PubMed]
  7. Falconi R, Renzulli T, Zaccanti F (2006) Survival and reproduction in Aeolosoma viride (Annelida, Aphanoneura). Hydrobiologia 564:95–99. https://doi.org/10.1007/s10750-005-1711-2 doi: 10.1007/s10750-005-1711-2. [DOI]
  8. Bely AE, Sikes JM (2010) Latent regeneration abilities persist following recent evolutionary loss in asexual annelids. Proc Natl Acad Sci U S A 107:1464–1469. https://doi.org/10.1073/pnas.0907931107 doi: 10.1073/pnas.0907931107. [DOI] [PMC free article] [PubMed]
  9. Zattara EE, Bely AE (2016) Phylogenetic distribution of regeneration and asexual reproduction in Annelida: regeneration is ancestral and fission evolves in regenerative clades. Invertebr Biol 135:400–414. https://doi.org/10.1111/ivb.12151 doi: 10.1111/ivb.12151. [DOI]
  10. Chen CF, Sung TL, Chen LY et al (2018) Telomere maintenance during anterior regeneration and aging in the freshwater annelid Aeolosoma viride. Sci Rep 8:18078. https://doi.org/10.1038/s41598-018-36396-y doi: 10.1038/s41598-018-36396-y. [DOI] [PMC free article] [PubMed]
  11. Fok SKW, Chen CP, Tseng TL et al (2020) Caspase dependent apoptosis is required for anterior regeneration in Aeolosoma viride and its related gene expressions are regulated by the Wnt signaling pathway. Sci Rep 10:10692. https://doi.org/10.1038/s41598-020-64008-1 doi: 10.1038/s41598-020-64008-1. [DOI] [PMC free article] [PubMed]
  12. Gratzner HG (1982) Monoclonal antibody to 5-bromo- and 5-iododeoxyuridine: a new reagent for detection of DNA replication. Science 218:474–475. https://doi.org/10.1126/science.7123245 doi: 10.1126/science.7123245. [DOI] [PubMed]
  13. Salic A, Mitchison TJ (2008) A chemical method for fast and sensitive detection of DNA synthesis in vivo. Proc Natl Acad Sci U S A 105:2415–2420. https://doi.org/10.1073/pnas.0712168105 doi: 10.1073/pnas.0712168105. [DOI] [PMC free article] [PubMed]
  14. Buck SB, Bradford J, Gee KR et al (2008) Detection of S-phase cell cycle progression using 5-ethynyl-2′-deoxyuridine incorporation with click chemistry, an alternative to using 5-bromo-2′-deoxyuridine antibodies. Biotechniques 447:927–929. https://doi.org/10.2144/000112812 doi: 10.2144/000112812. [DOI] [PubMed]
  15. Planques A, Malem J, Parapar J et al (2019) Morphological, cellular and molecular characterization of posterior regeneration in the marine annelid Platynereis dumerilii. Dev Biol 445:189–210. https://doi.org/10.1016/j.ydbio.2018.11.004 doi: 10.1016/j.ydbio.2018.11.004. [DOI] [PubMed]

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