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. 2022;2453:261–278. doi: 10.1007/978-1-0716-2115-8_15

AIRR Community Guide to Planning and Performing AIRR-Seq Experiments.

Anne Eugster, Magnolia L Bostick, Nidhi Gupta, Encarnita Mariotti-Ferrandiz, Gloria Kraus, Wenzhao Meng, Cinque Soto, Johannes Trück, Ulrik Stervbo, Eline T Luning Prak; AIRR Community
PMCID: PMC9761540  PMID: 35622331

Abstract

The development of high-throughput sequencing of adaptive immune receptor repertoires (AIRR-seq of IG and TR rearrangements) has provided a new frontier for in-depth analysis of the immune system. The last decade has witnessed an explosion in protocols, experimental methodologies, and computational tools. In this chapter, we discuss the major considerations in planning a successful AIRR-seq experiment together with basic strategies for controlling and evaluating the outcome of the experiment. Members of the AIRR Community have authored several chapters in this edition, which cover step-by-step instructions to successfully conduct, analyze, and share an AIRR-seq project.

Full text of this article can be found in Bookshelf.

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