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. 2022;2450:263–291. doi: 10.1007/978-1-0716-2172-1_14

Studying Echinodermata Arm Explant Regeneration Using Echinaster sepositus.

Cinzia Ferrario, Yousra Ben Khadra, Michela Sugni, M Daniela Candia Carnevali, Pedro Martinez, Francesco Bonasoro
PMCID: PMC9761906  PMID: 35359313

Abstract

Echinoderms are marine invertebrate deuterostomes known for their amazing regenerative abilities throughout all life stages. Though some species can undergo whole-body regeneration (WBR), others exhibit more restricted regenerative capabilities. Asteroidea (starfish) comprise one of the few echinoderm taxa capable of undergoing WBR. Indeed, some starfish species can restore all tissues and organs not only during larval stages, but also from arm fragments as adults. Arm explants have been used to study cells, tissues and genes involved in starfish regeneration. Here, we describe methods for obtaining and studying regeneration of arm explants in starfish, in particular animal collection and husbandry, preparation of arm explants, regeneration tests, microscopic anatomy techniques (including transmission electron microscopy, TEM) used to analyze the regenerating explant tissues and cells plus a downstream RNA extraction protocol needed for subsequent molecular investigations.

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References

  1. Wilkie IC (2005) Mutable collagenous tissue: overview and perspectives. In: Matranga V (ed) Echinodermata. Progress in molecular and subcellular biology. Marine molecular biotechnology, vol 5. Springer, Berlin, pp 221–250 doi: 10.1007/3-540-27683-1_10. [DOI] [PubMed]
  2. Emson RH, Wilkie IC (1980) Fission and autotomy in echinoderms. Ocean Mar Biol Ann Rev 18:155–250
  3. Ruppert EE, Fox RS, Barnes RD (2004) Invertebrate zoology: a functional evolutionary approach. Thomson Brooks/Cole, Belmont
  4. Cortés Rivera Y, Hernández RI, Martin S, del Angel P et al (2016) Regenerative potential of the sea star Linckia guildinguii. Hidrobiológica 26(1):95–100
  5. Thorndyke MC, Chen W-C, Beesley PW et al (2001) Molecular approach to echinoderm regeneration. Microsc Res Tech 55:474–485 doi: 10.1002/jemt.1192. [DOI] [PubMed]
  6. Hinman VF, Nguyen AT, Cameron RA et al (2003) Developmental gene regulatory network architecture across 500 million years of echinoderm evolution. Proc Natl Acad Sci 100(23):13356–13361 doi: 10.1073/pnas.2235868100. [DOI] [PMC free article] [PubMed]
  7. Czarkwiani A, Ferrario C, Dylus DV et al (2016) Skeletal regeneration in the brittle star Amphiura filiformis. Front Zool 13:18 doi: 10.1186/s12983-016-0149-x. [DOI] [PMC free article] [PubMed]
  8. Ben Khadra Y, Sugni M, Ferrario C et al (2017) An integrated view of asteroid regeneration: tissues, cells and molecules. Cell Tissue Res 370(1):13–28 doi: 10.1007/s00441-017-2589-9. [DOI] [PubMed]
  9. Ferrario C, Ben Khadra Y, Czarkwiani A et al (2018) Fundamental aspects of arm repair phase in two echinoderm models. Dev Biol 433(2):297–309 doi: 10.1016/j.ydbio.2017.09.035. [DOI] [PMC free article] [PubMed]
  10. Gildor T, Cary GA, Lalzar M et al (2019) Developmental transcriptomes of the sea star, Patiria miniata, illuminate how gene expression changes with evolutionary distance. Sci Rep 9:16201 doi: 10.1038/s41598-019-52577-9. [DOI] [PMC free article] [PubMed]
  11. Candia Carnevali MD, Bonasoro F, Patruno M et al (1998) Cellular and molecular mechanisms of arm regeneration in crinoid echinoderms: the potential of arm explants. Dev Genes Evol 208:421–430 doi: 10.1007/s004270050199. [DOI] [PubMed]
  12. Bonasoro F, Candia Carnevali MD, Sala F et al (1999) Regenerative potential of crinoid arm explants. In: Candia Carnevali MD, Bonasoro F (eds) Echinoderm research 1998. Balkema, Rotterdam, Brookfield, pp 133–138
  13. Candia Carnevali MD, Bonasoro F (2001) Microscopic overview of crinoid regeneration. Microsc Res Tech 55:403–426 doi: 10.1002/jemt.1187. [DOI] [PubMed]
  14. Candia Carnevali MD (2006) Regeneration in echinoderms: repair, regrowth and cloning. Invert Surviv J 3:64–76
  15. Ben Khadra Y, Sugni M, Ferrario C et al (2018) Regeneration in stellate echinoderms: Crinoidea, Asteroidea and Ophiuroidea. Results Probl Cell Differ 65:285–320 doi: 10.1007/978-3-319-92486-1_14. [DOI] [PubMed]
  16. Valoti G, Ferrario C, Candia Carnevali MD et al (2016) Regenerative potential of Echinaster sepositus (Retzius, 1783) arm explants: macro- and microscopic analyses. In: Proceedings of the LXXVII National Congress of the Italian Zoological Union (UZI)
  17. Villamor A, Becerro MA (2010) Matching spatial distributions of the sea star Echinaster sepositus and crustose coralline algae in shallow rocky Mediterranean communities. Mar Biol 157:2241–2251
  18. Ben Khadra Y, Ferrario C, Di Benedetto C et al (2015) Wound repair during arm regeneration in the red starfish Echinaster sepositus. Wound Repair Regen 23:611–622 doi: 10.1111/wrr.12333. [DOI] [PubMed]
  19. Ben Khadra Y, Ferrario C, Di Benedetto C et al (2015) Re-growth, morphogenesis, and differentiation during starfish arm regeneration. Wound Repair Regen 23:623–634 doi: 10.1111/wrr.12336. [DOI] [PubMed]
  20. Ben Khadra Y, Dimassi N, Martinez P (2018) Expression of Wnt genes in Echinaster sepositus arm regeneration. Int J Biochem Physiol 3(1):1–10
  21. Ben Khadra Y, Said K, Thorndyke M, Martinez P (2014) Homeobox genes expressed during echinoderm arm regeneration. Biochem Genet 52(3–4):166–180 doi: 10.1007/s10528-013-9637-2. [DOI] [PubMed]
  22. Gabre JL, Martinez P, Sköld HN, Ortega-Martinez O, Abril JF (2015) The coelomic epithelium transcriptome from a clonal sea star, Coscinasterias muricata. Mar Genomics 3:245–248 doi: 10.1016/j.margen.2015.07.010. [DOI] [PubMed]

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