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. 2022;2533:71–80. doi: 10.1007/978-1-0716-2501-9_5

Structural Studies of Eukaryotic RNA Polymerase I Using Cryo-Electron Microscopy.

Michael Pilsl, Christoph Engel
PMCID: PMC9761920  PMID: 35796983

Abstract

Technical advances have pushed the resolution limit of single-particle cryo-electron microscopy (cryo-EM) throughout the past decade and made the technique accessible to a wide range of samples. Among them, multisubunit DNA-dependent RNA polymerases (Pols) are a prominent example. This review aims at briefly summarizing the architecture and structural adaptations of Pol I, highlighting the importance of cryo-electron microscopy in determining the structures of transcription complexes.

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