Table 4.
Determination of Kb and n values for all substances with DNA/BSA at pH of 7.4 using spectrofluorometer.
| Compounds | DNA-/BSA-binding constants | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| SV methods for determining DNA-binding characteristics (SV methods for determining BSA-binding characteristics)∗ | LWB method KLB × 104 M−1 | Scatchard analysis | K app × 107 M−1 | |||||||
| Method I | Method II | |||||||||
| K q × 1012 M−1s−1 | K SV × 104 M−1 | K ass × 104 M−1 | n | ∆Gb° (kJM−1) | P | K SA × 104 M−1 | n | |||
| (HL) | 1.1636 (2.639) | 1.1636 (2.639) | 0.9606 (1.062) | 0.973 (0.926) | −22.720 (−23.0) | 0.0900 (0.464) | 0.6985 | 2.3194 | 1.096 | 0.5829 |
| (1a) | 5.2474 (8.691) | 5.2474 (8.691) | 5.0564 (8.734) | 1.168 (1.059) | −26.835 (−28.2) | 0.2000 (0.252) | 1.8846 | 3.3151 | 1.033 | 0.9894 |
| (2a) | 3.2694 (4.805) | 3.2694 (4.805) | 1.7368 (5.291) | 1.055 (1.041) | −24.187 (−26.9) | 0.1832 (0.348) | 1.5630 | 3.0190 | 0.971 | 0.6569 |