Key resources table.
| Reagent type(species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (M. musculus) | Osr2Cre | Rulang Jiang, Cincinnati Children’s Hospital; Chen et al., 2009 | ||
| Strain, strain background (M. musculus) | Tgfbr1fl/fl | Dudas et al., 2006; Larsson et al., 2001 | ||
| Strain, strain background (M. musculus) | Fgf18fl/fl | David Ornitz, Washington University School of Medicine; Hagan et al., 2019 | ||
| Strain, strain background (M. musculus) | Rosa26LSL-tdTomato | Jackson Laboratory; Madisen et al., 2010 | Stock No. 007905 RRID:IMSR_JAX:007905 |
|
| Strain, strain background (M. musculus) | C57BL/6J | Jackson Laboratory | Stock No. 000664 RRID:IMSR_JAX:000664 |
|
| Sequence-based reagent | RNAScope Probe-Mm-Aldh1a2 | Advanced Cell Diagnostics | Cat# 447391 | |
| Sequence-based reagent | RNAScope Probe-Mm-Tbx15 | Advanced Cell Diagnostics | Cat# 558761 | |
| Sequence-based reagent | RNAScope Probe- Mm-Smoc2 | Advanced Cell Diagnostics | Cat# 318541 | |
| Sequence-based reagent | RNAScope Probe-Mm-Fgf18 | Advanced Cell Diagnostics | Cat# 495421 | |
| Sequence-based reagent | RNAScope Probe-Mm-Lama4 | Advanced Cell Diagnostics | Cat# 494901 | |
| Sequence-based reagent | RNAScope Probe- Mm-Fgfr1 | Advanced Cell Diagnostics | Cat# 443491 | |
| Sequence-based reagent | RNAScope Probe- Mm-Fgfr2 | Advanced Cell Diagnostics | Cat# 443501 | |
| Sequence-based reagent | RNAScope Probe- Mm-Fgfr3 | Advanced Cell Diagnostics | Cat# 440771 | |
| Sequence-based reagent | RNAscope Probe- Mm-Fgfr4 | Advanced Cell Diagnostics | Cat# 443511 | |
| Sequence-based reagent | RNAScope Probe- Mm-Myf5 | Advanced Cell Diagnostics | Cat# 492911 | |
| Sequence-based reagent | RNAScope Probe-Mm-Hic1 | Advanced Cell Diagnostics | Cat# 464131 | |
| Sequence-based reagent | RNAScope Probe-Mm-Creb5 | Advanced Cell Diagnostics | Cat# 572891 | |
| Sequence-based reagent | RNAScope Probe-Mm-Meox1-C2 | Advanced Cell Diagnostics | Cat# 530641-C2 | |
| Sequence-based reagent | RNAScope Probe-Mm-Etv5 | Advanced Cell Diagnostics | Cat# 316961 | |
| Sequence-based reagent | RNAScope Probe-Mm-Bach2-C3 | Advanced Cell Diagnostics | Cat# 887121-C3 | |
| Sequence-based reagent | RNAScope Probe-Mm-Myod1 | Advanced Cell Diagnostics | Cat# 316081 | |
| Sequence-based reagent | RNAScope Probe- Mm-Myod1-C2 | Advanced Cell Diagnostics | Cat# 316081-C2 | |
| Sequence-based reagent | BaseScope Probe- BA-Mm-Fgf18-3zz-st-C1 | Advanced Cell Diagnostics | Cat# 1118021-C1 | |
| Antibody | Mouse monoclonal, Myosin heavy chain (MHC) | DSHB | Cat# MF20 | (1:25) |
| Antibody | Rabbit polyclonal, RFP | Rockland | Cat# 600-401-379 | (1:500) |
| Antibody | Rabbit monoclonal, pSmad2 | Cell Signaling Technology | Cat# 3108 RRID:AB_490941 |
(1:500) |
| Antibody | Rabbit monoclonal, MyoD | Abcam | Cat# ab133627 RRID:AB_2890928 |
(1:200) |
| Antibody | Rat monoclonal, BrdU | Abcam | Cat# ab6326 RRID: AB_305426 |
(1:100) |
| Antibody | Rabbit Polyclonal, Cleaved Caspase-3 | Cell Signaling Technology | Cat# 9661 RRID: AB_2341188 |
(1:100) |
| Antibody | Rabbit monoclonal, Smad2/3 | Cell Signaling Technology | Cat# 8685 RRID:AB_10889933 |
(1:20) |
| Antibody | Rabbit monoclonal, IgG | Cell Signaling Technology | Cat# 3900 RRID:AB_1550038 |
(1:20) |
| Antibody | Goat polyclonal anti-Mouse Alexa Fluor 488 | Life Technologies | Cat# A-11001 RRID:AB_2534069 |
(1:200) |
| Antibody | Goat polyclonal anti-Rat Alexa Fluor 488 | Life Technologies | Cat# A-11006 RRID:AB_141373 |
(1:200) |
| Antibody | Goat polyclonal anti-Rabbit Alexa Fluor 488 | Life Technologies | Cat# A-11008 RRID:AB_143165 |
(1:200) |
| Antibody | Goat polyclonal anti-Rabbit Alexa Fluor 568 | Life Technologies | Cat# A-11036 RRID:AB_10563566 |
(1:200) |
| Antibody | Goat polyclonal anti-rabbit IgG Antibody (H+L), HRP |
Vector Laboratories | Cat# PI-1000 RRID:AB_2336198 |
(1:200) |
| Peptide, recombinant protein | Recombinant human TGF-β1 | R&D Systems | Cat# 7754-BH | |
| Peptide, recombinant protein | Recombinant human FGF18 | Peprotech | Cat# 100–28 | |
| Cell line (Mus musculus) | C2C12 | ATCC | Cat# CRL-1772 RRID:CVCL_0188 |
|
| Commercial assay or kit | RNAScope Multiplex Fluorescent Kit v2 | Advanced Cell Diagnostics | Cat# 323110 | |
| Commercial assay or kit | BaseScope detection reagent kit v2-Red | Advanced Cell Diagnostics | Cat# 323910 | |
| Commercial assay or kit | TSA Plus Cyanine 3 System | Perkin Elmer | Cat# NEL744001KT | |
| Commercial assay or kit | TSA Plus Fluoresceine System | Perkin Elmer | Cat# NEL771B001KT | |
| Commercial assay or kit | RNeasy Micro Kit | QIAGEN | Cat# 74004 | |
| Commercial assay or kit | iScript Advanced cDNA Synthesis Kit | Bio-Rad | Cat# 1725038 | |
| Commercial assay or kit | SsoFast EvaGreen Supermix | Bio-Rad | Cat# 1725201 | |
| Commercial assay or kit | Chromium single-cell 3’ v2 reagent kit | 10x Genomics | Cat# PN-120267 | |
| Commercial assay or kit | Cut and Run assay kit |
Cell Signaling | Cat# 86652 | |
| Software, algorithm | Cell ranger | 10x Genomics.Inc | RRID:SCR_017344 | |
| Software, algorithm | Seurat | Satija lab | RRID:SCR_016341 | |
| Software, algorithm | CellChat | Jin Lab | ||
| Software, algorithm | SCENIC | Aerts lab | RRID:SCR_017247 | |
| Software, algorithm | GraphPad Prism | GraphPad Software | RRID:SCR_002798 |