Figure 7.
Impact of OMA treatment on the BBB permeability and viral load in the gNVU in the context of VEEV TrD infection. (A) gNVUs that were infected (TrD[MOI:0.1]), untreated (n = 3) or infected and treated with OMA (0.5 µM) (n = 3) were assessed for endothelial permeability status by quantification of FITC-dextran in the brain and vascular chambers. Permeability is depicted as variables of porous membrane units and time (cms/s2) and is the average of three independent data points for each time point represented; (B,C) the perfused media obtained from the brain and vascular compartments of the infected, untreated and infected treated gNVUs at 48- and 72-h post infection were used to quantify infectious viral titer by plaque assay. Data represent average of three independent samples and titers are expressed as PFU/mL. Statistical analysis was carried out using t-test * p < 0.05.