TABLE 2.
Activation Z‐scores of the upstream drivers of the Th2/cell cycle and IFN‐associated modules at T0/T1/T3 in remission/SU and allergic
| Remission/SU | Allergic | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Th2+ cell cycle | IFN | Th2+ cell cycle/IFN | Th2+ cell cycle/IFN | Th2+ cell cycle | IFN | |||||
| T0 | T0 | T1 | T3 | T0 | T1 | T3 | T0 | T1 | T3 | |
| Th1 | ||||||||||
| IFNG | 2.52 | 8.06 | 8.75 | 7.40 | 2.90 | ns | 7.31 | 2.70 | 8.84 | 4.40 |
| Th2 | ||||||||||
| IL4 | 5.56 | ns | ns | 2.19 | 5.86 | 4.02 | 3.66 | ns | ns | ns |
| IL5 | 4.70 | 4.37 a | 4.42 a | 4.26 a | 4.99 | 3.61 | 5.35 | ns | 3.69 | ns |
| IL9 | ns | ns | 2.11 | ns | N/A | 2.62 | 2.53 | ns | ns | ns |
| IL13 | 2.00 | ns | ns | ns | 3.10 | 2.45 | 2.56 | ns | ns | ns |
| Interferons type 1 and 3 | ||||||||||
| IFNA2 | 3.18 | 7.16 | 7.82 | 6.57 | 2.52 | ns | 6.07 | 2.54 | 7.77 | 4.28 |
| IRF7 | ns | 7.16 | 7.33 | 6.83 | ns | ns | 6.10 | 3.35 | 7.00 | 3.90 |
| STAT1 | ns | 6.13 | 6.88 | 6.61 | 2.11 | ns | 5.83 | 3.28 | 6.84 | 3.22 |
| IFNA group | 2.72 | 6.56 | 7.24 | 6.52 | ns | ns | 6.01 | 2.29 | 7.30 | 3.40 |
| IFNL1 | ns | 5.76 | 6.33 | 5.25 | ns | ns | 4.46 | 2.10 | 6.52 | 4.05 |
| T cell associated | ||||||||||
| CD3 complex | 7.04 | 2.92 | 5.91 | 5.64 | 6.25 | 5.31 | 6.74 | ns | 4.10 | ns |
| CD28 | 6.09 | ns | 5.10 | 5.48 | 5.79 | 4.53 | 5.59 | ns | 2.94 | ns |
| TCR complex | 4.85 | ns | 5.97 | 4.86 | 4.26 | 3.35 | 5.45 | ns | 3.34 | ns |
| IL2 | 5.11 | 3.64 | 5.40 | 5.44 | 5.11 | 4.16 | 5.78 | ns | 4.05 | ns |
| CSF2 | 6.39 | 2.95 | 5.84 | 6.17 | 5.62 | 4.95 | 6.83 | ns | 3.44 | ns |
| CD40LG | 4.64 | 2.91 | 5.22 | 3.99 | 3.77 | 2.74 | 5.32 | ns | 3.86 | ns |
| Growth factors/cell cycle | ||||||||||
| ERBB2 | 6.16 | ns | 3.83 | 3.72 | 6.06 | 4.29 | 4.84 | ns | ns | ns |
| MYC | 5.58 | ns | 2.89 | 2.79 | 6.27 | 3.48 | 4.50 | ns | ns | ns |
| EGF | 4.99 | ns | ns | 3.75 | 4.63 | 4.51 | 4.86 | ns | ns | ns |
| EGFR | 4.00 | ns | 2.59 | ns | 4.15 | 3.52 | 4.31 | ns | ns | ns |
| Inflammatory | ||||||||||
| TNF | 6.60 | 6.17 | 7.40 | 7.62 | 5.38 | 5.04 | 7.58 | ns | 6.26 | 3.32 |
| IL1B | 5.60 | 4.82 | 6.57 | 5.86 | 5.16 | 3.37 | 6.67 | 2.60 | 5.74 | 2.55 |
| NFkB complex | 5.48 | 4.35 | 5.63 | 5.56 | 5.29 | 3.61 | 6.51 | 2.17 | 4.73 | ns |
Putative regulators of the modules were identified at each timepoint T0/T1/T3 employing Upstream Regulator Analysis. Molecular drivers with Benjamini & Hochberg adjusted p < 0.05 and absolute Z‐scores >2.0 were deemed significant. Positive values indicate activation, and negative values indicate inhibition.
Bold text highlights z‐scores for Th1 INFG, type 1 and 3 interferons and Th2.
Abbreviation: ns, not significant.
a
Z‐score ranking for IL5 is > top 30.