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. 2022 Dec 6;26(1):105733. doi: 10.1016/j.isci.2022.105733

Figure 3.

Figure 3

Identification of SPC subsets in buffalo

(A) Re-clustering tSNE plot of SPC subsets in buffalo testes.

(B) Multi-volcano plot showing meiotic genes (test.use = “wilcox”, min.pct = 0.65) in pairwise comparisons in all cell types. The graph displayed the top five annotated genes with the most fold change for each group.

(C) Feature plots of representative marker genes for pre.L, L, Z, eP, mP, lP, D, and MI-II.

(D) IF analysis of antibody staining against SYCP3 on paraffin sections of prepubertal and pubertal buffalo testes. Scale bar, 20 μm.

(E) Pseudotime trajectories of buffalo SPC subsets, colored by cell type (left) and pseudotime value (right).

(F) Heatmap showing DEGs (q-value < 0.01) expression (rows) along the pseudotime (columns) axis.

(G) Feature plots, trajectory plots, and pseudotime trajectory expression patterns of selected genes in pre.L, L, Z, eP, mP, lP, D, and MI-II.