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. 2022 Jun 30;72(1):71–84. doi: 10.2337/db21-1086

Figure 3.

Figure 3

Blocking activity of anti-PLIN1 autoantibodies. A: Detection of blocking activity of anti-PLIN1 autoantibodies using serum samples at 1/250 dilution incubated with a fixed amount (100 ng) of recombinant PLIN1. The unspecific blocking activity (8.31%) was established using the mean value obtained for 50 healthy donors. B: Dose-dependent inhibition of the interaction of PLIN1 and ABHD5 in the presence of sera samples from anti-PLIN1–positive patients (AGL3, AGL13, AGL33, and AGL39). Serum from AGL19 and pooled serum from five healthy donors were negative for anti-PLIN1 autoantibodies. Serially diluted serum samples were mixed with a fixed amount (100 ng) of recombinant PLIN1. For both images, results were expressed as mean ± SD from two independent experiments.