Table 1.
Specific forward and reverse primers (from Macrogen) designed for oxidative stress, inflammation and apoptosis-related genes (SOD1, GPx1, CAT, Nrf-2, ICAM-1 and Akt1), and for reference endogenous genes (ß- Actin and TBP) in rat liver.
| Symbols | Genes Rattus norvegicus | Primer Sequences (Forward and Reverse):5’→3’ |
|---|---|---|
| SOD1 | Superoxide dismutase 1 | F: CCACTGCAGGACCTCATTTT R: CACCTTTGCCCAAGTCATCT |
| GPx1 | Glutathione peroxidase 1 | F: ATAGAAGCCCTGCTGTCCAA R: GAAACCGCCTTTCTTTAGGC |
| CAT | Catalase | F: ACATGGTCTGGGACTTCTGG R: CAAGTTTTTGATGCCCTGGT |
| Nrf-2 | Nuclear factor-erythroid derived 2-like 2 | F: CCTAAAGCACAGCCAACACA R: GCCTCTAATCGGCTTGAATG |
| Akt1 | Protein kinase B | F: CCTCAAGAATGATGGCACCT R: TTTGAGTCCATCAGCCACAG |
| ICAM-1 | Intercellular adhesion molecule 1 | F: AGGTATCCATCCATCCCACA R: GCCACAGTTCTCAAAGCACA |
| ß-Actin | ß-actin | F: GGGTATGGAATCCTGTGGCATCC R: GCTCAGGAGGAGCAATGATCTTGA |
| TBP | TATA box binding protein | F: GACTCCTGTCTCCCCTACCC R: CTCAGTGCAGAGGAGGGAAC |