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. 2022 Dec 5;26(1):105737. doi: 10.1016/j.isci.2022.105737
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Anti-Caspase 3 BD Bioscience RRID: AB_397274; Cat#559565
Donkey Anti-Rabbit Alexa Fluor 568 Life Technologies RRID: AB_2534017; Cat#A10042
Anti-Digoxigenin Sigma Aldrich RRID: AB_2734716; Cat#11093274910
Anti-Flag M2 Sigma Aldrich RRID: AB_259529; Cat#F3165
HRP-Donkey Anti-Mouse IgG Jackson Immunoresearch RRID: AB_2340770; Cat#715-035-150
Anti-Phospho-Histone H3 Cell Signaling Technology RRID: AB_331535; Cat#9701

Chemicals, peptides, and recombinant proteins

SNAG peptide GenScript N/A
Proteinase K Sigma Aldrich Cat#RPROTK-RO
Ambion SP6 RNA Polymerase Invitrogen Cat#AM2071
Ambion T7 RNA Polymerase Invitrogen Cat#AM2082
Ribonucleic Acid Type VI Sigma Aldrich Cat#R6625
Heparin Sigma Aldrich Cat#H3393
Maleic Acid Sigma Aldrich Cat#M0375
Blocking Reagent Roche Cat#11096176001
Fetal Bovine Serum, Heat Inactivated Gibco Cat#10438026
RNAlater Qiagen Cat#76104
Phusion High-Fidelity DNA Polymerase NEB Cat#M0530S
Protease Inhibitor Cocktail Sigma Aldrich Cat#P8340
gfi1b sgRNA This paper N/A

Critical commercial assays

OneStep RT-PCR Qiagen Cat#210210
QuikChange II Site-Directed Mutagenesis Kit Agilent Cat#200523
QIAquick Gel Extraction Kit Qiagen Cat#28704
pGEM-T-Easy Vector Systems Promega Cat#A1360
LightScanner Master Mix BioFire Defense Cat#HRLS-ASY-0003
mMessage mMachine SP6 Transcription Kit Invitrogen Cat#AM1340
NucAway Spin Columns Thermo Fisher Cat#AM10070
DIG RNA Labeling Mix Sigma Aldrich Cat#11277073910
BCIP/NBT Substrate Kit Vector Laboratories Cat#SK5400
PureLink RNA MicroKit Invitrogen Cat#12183016
SuperSignal West Femto Maximum Sensitivity Substrate Thermo Fisher Cat#34095
Cas9 IDT Cat#1081058
HiScribe T7 Quick High Yield RNA Synthesis RNA Kit NEB Cat#E2050S
RNeasy Plus Mini Kit Qiagen Cat#74134
Protease inhibitor cocktail Sigma Aldrich Cat#P8340
PVDF membrane Millipore Cat#IPVH00010
SuperSignal West Femto Maximum Sensitivity Substrate Thermo Fisher Cat#34095
Autoradiography film Genesee Scientific Cat#30-507

Deposited data

Raw data This paper GEO: GSE198476

Experimental models: Organisms/strains

Zebrafish: kdm1a 23095 ZFIN: ZDB-ALT-131217-16908
Zebrafish: kdm1a 36433 ZFIN: ZDB-ALT-161003-15414
Zebrafish: gfi1aa 16850 ZFIN: ZDB-ALT-131217-13025
Zebrafish: gfi1ab 40706 ZFIN: ZDB-ALT-160601-6870
Zebrafish: plrg1 hi3174aTg ZFIN: ZDB-ALT-040826-6
Zebrafish: gfi1b Δ17 This paper N/A

Oligonucleotides

Primers for genotyping, see Table S2 This paper N/A
Primers for cDNA amplification, see Table S2 This paper N/A
Primers for site directed mutagenesis, see Table S2 This paper N/A

Recombinant DNA

pUC57-zlsd1-3XFLAG This paper N/A
pUC57-zlsd1-K661A This paper N/A
pUC57-zlsd1-ΔQ632-3XFLAG This paper N/A
pUC57-zlsd1-D555K-3XFLAG This paper N/A
pUC57-zlsd1-A539E-3XFLAG This paper N/A
pUC57-zlsd1-ΔTower-3XFLAG This paper N/A
pUC57-zlsd1-ΔAOLC This paper N/A
pUC57-hLsd1-3XFLAG This paper N/A
pUC57-hLsd1-ΔAOLC This paper N/A
pGEM-T-Easy-gfi1b This paper N/A
pGEM-T-Easy-gfi1aa This paper N/A
pGEM-T-Easy-gfi1ab This paper N/A
pGEM-T-Easy-fli1a This paper N/A
pBluescript-gata1 Detrich et al.76 N/A
pUC57-etv2 Sumanas et al.77 N/A
pBK-snail1a Thisse et al.78 N/A
pBK-snail1b Thisse et al.79 N/A
pBS-IISK-snai2 Thisse et al.80 ZFIN: ZDB-EST-030328-40

Software and algorithms

ImageJ
LightScanner Call-IT Idaho Technology Inc.
Adobe Illustrator Adobe
Adobe Photoshop Adobe
Integrated Genomics Viewer Broad Institute
RStudio RStudio
Pymol Schrodinger Inc.
GraphPad Prism Dotmatics