Plant Cell (2022) 34: 3006-3027 doi: 10.1093/plcell/koac147
Yu Chen, Xiayan Liu, Wenjing Zhang, Jie Li, Haofeng Liu, Lan Yang, Pei Lei, Hongchang Zhang, Fei Yu
Author for correspondence: feiyu@nwafu.edu.cn
The authors were made aware of mistakes in the preparation of Figure 6J, namely, an image for the input was mistakenly used as the pulldown result. Images of immunoblotting were obtained with a Bio-Rad ChemiDoc machine using Image Lab software (Bio-Rad). For each blot, we usually took a number of images with various exposure times. When we were preparing images for Figure 6J, an anti-MBP immunoblotting image for the input was mistakenly used for the pull-down panel. We sincerely apologize for this mistake and would like to issue the corrected image below. Uncropped raw images for Figure 6J are also presented. This correction does not alter any of the findings or conclusions presented in the manuscript.
In addition, concern was raised that for the pull-down assay (Figure 6J) and the Co-IP assay (Figure 6L), immunoblotting images for KTN1 were shown in separate boxes from those for MOR1-C578 (for the pull-down) or MOR1-GFP (for the Co-IP). In the pull-down assay, the input samples were loaded in duplicated sets on an SDS-PAGE gel. After running the gel, proteins were transferred onto a PVDF membrane. The membrane was then cut into two pieces, with each piece containing an identical set of input samples. One piece of the membrane was used for immunoblotting using anti-MBP to detect MBP and MBP-MOR1-C578, while the other piece of the membrane was used for immunoblotting using anti-His to detect His-KTN1. The pull-down samples were similarly run in duplicated sets, and the two pieces of membranes, with each piece containing an identical set of pull-down samples, were immunoblotted with anti-MBP to detect MBP and MBP-MOR1-C578 and anti-His to detect His-KTN1, respectively. The immunoblotting of the input and Co-IP samples in the Co-IP assay was carried out in a similar manner to the pull-down assay. It is our belief that Ponceau-stained membranes or gels are usually not included in the presentation of pull-down or Co-IP experiments in publications, so we didn’t include these images in Figure 6J and 6L.
Editors’ note: This correction notice was reviewed by members of The Plant Cell editorial board. The authors are responsible for providing a complete listing and accurate explanations for all known errors or instances of inappropriate data handling or image manipulation associated with the original publication.
Figure Legends
Figure 6.
MOR1 physically interacts with KTN1. J, In vitro pull-down of His-KTN1 by MBP-C578. MBP served as a negative control. MBP-C578 and MBP were detected by an anti-MBP antibody. His-KTN1 was detected by an anti-His antibody.
Source File 1. Uncropped raw image of anti-MBP immunoblot for the input used in Fig. 6J.
Source File 2. Uncropped raw image of anti-MBP immunoblot for the pull-down used in Fig. 6J.
Source File 3. Uncropped raw image of anti-His immunoblot for the input used in Fig. 6J.
Source File 4. Uncropped raw image of anti-His immunoblot for the pull-down used in Fig. 6J.
