Abstract
Representation of triple unsymmetrical micro ring resonators (TUMRR) with single input and single output waveguide has been executed in this paper. Statistical demonstration of the presented arrangement is realized in the z-domain by a delay line signal processing method. The transfer function of the triple unsymmetrical micro ring resonator is calculated by Mason’s Gain rule. The offered arrangement is performed on SOI (Silicon in Insulator) platform to achieve the filtering and biochemical sensing intentions. To acquire field response of the arrangement, Finite difference time domain (FDTD) technique is used. Characteristics of optical filters are studied from the frequency response plot and the achieved free spectral range is 243.5 GHz. The shift in wavelength for biosensing application is accomplished through the OptiFDTD software. The sensitivity of the proposed TUMRR based biosensor is around 200–280 nm/RIU for different blood cells where as the Q-factor attained is 1938.
Keywords: Biosensor, MicroRing resonator, Delay-line signal processing, Filter
Introduction
At present, several different models of multiple ring resonators having upgraded FSR with lower crosstalk, using the technique delay line signal processing and signal flow graph method were developed through many researches (Oda et al. 1991; Little et al. 1997a; Suzuki et al. 1995; Moslehi et al. 1984a; Mandal et al. 2006; Dey and Mandal 2012; Yanagase et al. 2002). Lakra et al. (2017), presented a single input and two output unsymmetrical MRR arrangements having a FSR of 446 THz. All the rings are unequal in size as well as nature in this offered paper. Ranjan et al. (2018a), presented a single input and three output arrangement with a triple optical numerous ring resonators having a FSR approximately 110 THz. Ranjan et al. (2017), offered a 2 × 2 input output arrangement with an unequal ring resonators having a FSR approximately 0.4 THz. FSR of the resonators are reciprocal of its radius so in order to increase the FSR, the radius resonators must be lower. Radius of ring resonators is restricted by bending loss.
This paper, offered a single input and single output waveguide which behaves as a biosensor to detect diseases cells from prescribed blood samples. In (Liu et al. 2020), an optical fiber SPR biosensor is proposed with MoSe2-Au structure for biosensing application with real-time detection, higher sensitivity and superior applicability in immunoassay. In (Guan and Huang 2019) an ultrasensitive optical microfiber-based biosensors is proposed where sensitivity and LOD have increased magnificently by increasing the light-matter interaction. In (Singh and Jha 2019), fabrication and validation of optical biosensor for non-invasive monitoring of glucose using saliva is proposed where the enzymatic reaction resulted in pH variation which was detected by electronic meter. In (Divagar et al. 2021), a dip type, wash free plasmonic fiber optic absorbance biosensor (P-FAB) is proposed for point-of-care detection of SARS-CoV-2 N-protein. In (Monfared et al. 2021), a highly sensitive quasi-D-shaped fiber optic biosensor is proposed for the detection of high refractive index liquid analytes via surface plasmon resonance. The proposed biosensor has a RI detection range of 0.15 RIU from 1.45 to 1.6. Various other optical biosensors having different shapes and characteristic were reported in Olyaee and Bahabady (2015), Tavousi et al. (2018), Jindal et al. (2016), Kim et al. (2008), Khozeymeh and Razaghi (2019).
The offered arrangement is designed through directionally coupled waveguide. The field investigation of the propagated wave is finished using OptiFDTD environment. This paper offers an expanded FSR which can be efficiently used in various communication platforms. The correlation between digital filters and optical waveguide were developed by Moslehi et al. (1984b), Jackson et al. (1985), has been exploited in this paper to calculate the transfer function of the offered micro ring resonator in z-domain. Mason's gain rule has been used to create a signal flow graph for non-identical ring resonators (Gad et al. 2011; D’azzo and Houpis 1995). There is various application of optical resonators like, laser resonator (McCall et al. 1992), add-drop filters (Little et al. 1997b), optical spectrum analyzers which measure and display the distribution power of an optical source over a specified wavelength span (Kalli and Jackson 1992), optical switching which amplifiers the optical signal (Vat et al. 2002), optical filter which allow the transmission of a specific wavelength (Razaghi and Laleh 2016) etc. The resonance state can be defined as (Madsen and Zhao 1999a);
| 1 |
where ring radius is R, group refractive index is ng, number of mode is “M” and resonant wavelength is “λ”. Optical ring resonator performs an important function to construct optical filters in communication systems whereas biosensors have numerous applications in field of medical, food production, ecosystems, security and aquatic etc.
The proposed micro ring resonator is basically developed to create an integrated sensor network. Through that the inherent filter characteristic will be an added advantage to transmit the sensor data in remote location. Several sensors working in different band with spacing of around 18–20 GHz can be integrated within this FSR. Therefore the proposed TUMRR having FSR of 243.5 GHz can accommodate roughly 10–15 sensor data i.e. 15 different sensors data can be transmitted to remote location using the filter characteristic of TUMRR.
Basic fundamental of optical filter
To accomplish the delay line signal processing, commonly unit delay is defined as, T = (Lu n)/c, where “Lu” refers to the unit delay length for the shortest path duration, valuable refractive index is “n” and speed of light is “c”. To achieve discrete signal, sampling a continuous signal at , where “n” refers sample numbers and “T” is for sampling space. Coupler method and delay length perform a crucial component for the valuable achievement of the optical filter. The frequency response of one period can be defined as free spectral range (FSR). The statistical correlation between FSR and unit delay is given by Ranjan and Mandal (2018b), FSR = 1/T = c/(n Lu), which is correlated with discrete impulse response. An integer multiple of unit delay is consider as total delay of filter. To demonstration, the z-transform of a discrete time signal f (z) is (Ranjan et al. 2016),
| 2 |
where z-transformed unit delay is z−n. Triple unsymmetrical micro ring resonator arrangements have four directional couplers that are drawn with a delay line signal processing method. One period of optical filters with discrete time delay is equal to FSR. To demonstrate, the Free Spectral Range in background of resonant wavelength (λ) is (Ranjan et al. 2016).
| 3 |
Vernier's concept is used to prevent the tiniest and smallest twisting radius, which result in the maximum loss. Each ring resonator's boundary has been changed such that it is now an integral multiple of the unit delay duration. With the use of discrete multiples of unit delay duration, locate the full optical loop. Vernier principle method is used to explain how to increase the Triple unsymmetrical Micro Ring Resonator's overall FSR. Vernier principle method is demonstrated as follows (Boeck et al. 2013).
| 4 |
where N, M and O are introduced as co-prime resonant numbers of the triple unsymmetrical micro ring resonator whose values are N = 6, M = 32 and O = 5 respectively. Radius of micro ring resonators are taken as R1 = 0.75 µm, R2 = 4 µm and R3 = 0.625 µm respectively.
Directional couplers
The optical couplers are a key component in the coupling and breaking of optical signals. The phenomenon is known as coupling and occurs when two waveguides are positioned close enough to one another that their evanescent fields overlap. The figure of directional optical coupler is given in Fig. 1, where , are input fields and , are output fields respectively. “C” is referred as through port transmission coefficient and “S” is referred as cross port transmission coefficient (Barbarossa et al. 1995).
Fig. 1.

Directional optical coupler
Here, “C” and “S” are referred as through port coupling coefficient and cross port coupling coefficient respectively. The coupling ratio is denoted by the symbol k, (Ranjan and Mandal 2018a)
| 5 |
where Pcoupled is referred as coupled power obtained from each coupler and Pinput is referred as power obtained from the same coupler. The block diagram of directional optical coupler is shown in Fig. 2. Transfer matrix presented in Eq. (6), where “q” (0 < q < 1) is referred to as amplitude transmission coefficient, also determines the correlation between the input and output field (Ranjan and Mandal 2018b).
| 6 |
Fig. 2.

Block representation of optical coupler
Transfer function of triple unsymmetrical micro ring resonator
Mason's gain rule and the signal flow graph approach are used to analyze the transfer function of the triple unsymmetrical micro ring resonators that are offered in the z-domain. The Tf is given in Eq. (7). (Mandal et al. 2006)
| 7 |
Figure 3 depicts the offered arrangement, which consists of four directional couplers and triple unsymmetrical micro ring resonators. The input and output ports are designated as X(z) and Y(z), respectively. Transfer function of given signal flow graph of offered arrangement is shown in Fig. 4.
Fig. 3.
Offered triple unsymmetrical micro ring resonator
Fig. 4.
Block diagram of proposed arrangement in z-domain
The offered TUMRR is shown in Fig. 3 as having a single input port and a single output port, X(z), and Y(z), respectively. According to the diagram in Fig. 3, the bus X (z) is coupled serially having coupling coefficient k1 and the bus Y(z) is coupled vertically with coupling coefficient k4. Due to the utilisation of triple unsymmetrical micro ring resonators, the offered arrangements suggest a wide FSR. The offered circuit's expanded FSR is utilised in communication networks to handle greater data.
The signal flow graph of the offered design in the z-domain is shown in Fig. 4. Here input port denotes the input signal and output port denotes the arrangement's output signal. Equation (8) provides an illustration of the transfer function (Tf) using Mason's gain rule, i.e. (Ranjan et al. 2016)
| 8 |
In this case, the ring's encircling expedition circulation loss is expressed as {ϒ = exp(− αL)} (Madsen and Zhao 1999b), where "L" stands for the ring's span and "α" for the amplitude reduction coefficient resulting from the loss purpose, material absorption, emission, and plane hardness, all losses combined. Therefore, Eq. (8) will be changed to Eq. 9 since the amplitude spread coefficient (q) is calculated as 1 (Li et al. 2008), i.e.
| 9 |
Characteristics of TUMRR
Ring resonator possess certain characteristics which must be satisfied for its application. Group delay and dispersion are important factors in filter application whereas quality factor and sensitivity are important factors for application as sensor.
Group delay
Group delay is defined as negative derivation of the phase of the transmittance with respect to the angular frequency (Ali et al. 2020), it can be termed as
| 10 |
where H(z) is referred as frequency response of the arrangement and Im{H(z)} and Re{H(z)} are imaginary and real part of frequency response. Slope of the phase with the frequency is termed as delay where it is being evaluated.
Dispersion
Derivative of group delay with respect to angular frequency termed as dispersion. It is the representation for deformation of signal in the region of resonant frequencies (Amoosoltani et al. 2021; Al Mahmod et al. 2018).
| 11 |
Quality factor
It is crucial for determining the free spectral range of the resonance state for any micro ring resonator. The Q-factor reveals the ability of a resonator to energy accumulation. The quality factor is the ratio of accumulated energy missing in a resonator arrangement. Q-factor can be determined as the sharpness of resonator crest in terms of full width half maxima (FWHM) and resonant frequency (Barbarossa et al. 1995).
| 12 |
Sensitivity
The ability to sense the smallest change of refractive index in a sensor is termed as sensitivity. Mathematically, it can be termed as the ratio of change in refractive index in nm to change in the refractive index solution flowing on the top of the surface of sensor. It is articulated as nm/RIU where RIU is the refractive index unit (Madsen and Zhao 1999b; Li et al. 2008).
| 13 |
Simulation results as optical filter
The frequency response analysis of the proposed TUMRR is done in MATLAB environment while its filed assessment is conceded out using OptiFDTD software. The micro ring perimeter sizes are taken as 4.71 µm, 25.12 µm and 3.925 µm. The corresponding co-prime resonant numbers are N = 6, M = 32 and O = 5. The unit delay length is 0.785. The ring loss is taken as 0.1 dB/cm and the amplitude of transmission coefficient taken as 1. The optimal coupling coefficient is taken as k1 = k2 = 0.40, k3 = k4 = 0.45. The FSR determined is 243.5 GHz. The frequency response plot along with its group delay and dispersion behavior is shown in Fig. 5. Table 1 represents the enhanced FSR of proposed configuration for same class of microring resonators. The detemined values of waveguides gap for the corsponding coupling coefficient is shown in Table 2.
Fig. 5.

Transmittance, Group delay and Dispersion plot of TUMRR
Table 1.
Parameters comparison obtained using vernier principle and without vernier principle
| Technique/Parameters | FSR (GHz) |
|---|---|
| TUMRR (Vernier principle) | 243.5 |
| Sanjoy Mandal et al. (2006) | 200 |
Table 2.
Calculated values of different coupling coefficient and the corresponding waveguide gap
| S.L. No | Coupling coefficient | Gap (nm) |
|---|---|---|
| k1 | 0.40 | 0.62 |
| k2 | 0.40 | 0.75 |
| k3 | 0.45 | 0.60 |
| k4 | 0.45 | 0.78 |
Validation of TUMRR as optical biosensor
The offered TUMRR biosensor was contoured with the measurement adjustment, such as tunable laser, optical power meter, polarization beam splitter and a polarizer. The SOI wafer, which is configured with a top silicon layer that is 200 nm thick and a silica buffer layer that is 2 µm thick, was utilized to model the TUMRR. When a fresh (healthy) blood cell is placed at the top layer of the TUMRR sensor, a FSR of 25 nm and full width half maxima (FWHM) of 8 nm were noticed which is shown in red color plot in Fig. 6. The Q-factor of the offered TUMRR is calculated and it is found to be 1938. To achieve the sensing application of the offered TUMRR configuration, different blood cells are applied on the top layer of the MRR. When any diseased blood sample is noticed, then the propagation of light will get disturbed, i.e. diseased blood samples occupying different refractive index. Placement of different blood sample gives freely shifts in resonant wavelength. The responsible elements which affect the shifting in resonant wavelength are bio-layer thickness, change of effective refractive index of the bond targets etc. As a result, when any diseased blood sample is located at the surface of the TUMRR, the resonant wavelength of the TUMRR moves from its initial location. The presence of different cells as compared to healthy cells is recognized via shifting in the resonant wavelength and few of them are presented in Table 3. The permittivity (ɛ) of cancer cells is proportional to the square of the refractive index (ɳ) (Ali et al. Feb. 2020; Amoosoltani et al. 2021);
Fig. 6.

Transmission spectra for a TUMRR for different cells along with their refractive indices where red represents normal blood cell, blue represent Jurkat cell and black represents MDA-MB-231 cells
Table 3.
Cancer cell with refractive index of sample (Ali et al. 2020);
| S.L. No | Name of cell | Disease | Refractive index |
|---|---|---|---|
| 1 | Normal (Healthy) | – | 1.35 |
| 2 | Jurkat | Leukemia | 1.39 |
| 3 | HeLa | Cervical cancer | 1.392 |
| 4 | PC-12 | Brain | 1.395 |
| 5 |
MDA- MB-231 |
Breast cancer | 1.399 |
| 6 | MCF-7 | Breast cancer | 1.401 |
In this paper, Jurkat cell which represents Leukemia and MDA-MB-231 cell which shows Breast Cancer is placed on the TUMRR surface to study the effectiveness as well as sensitivity of the offered biosensor. Jurkat cell leads to a shift of 8 nm while MDA-MB-231 leads to a shift of 14 nm with respect to healthy blood sample which is illustrated in Fig. 6. Hence, the calculated sensitivity of the offered TUMRR in case of Jurkat is 200 nm/RIU and in case of MDA-MB-231 it is computed as 285.71 nm/RIU. As discussed in (Ciminelli et al. 2013),sensitivity of micro-ring resonator is classified as:
Device Sensitivity
Waveguide Sensitivity
The device sensitivity depends only on the device properties, while the waveguide sensitivity depends on the waveguide structure. The design strategy is to optimize these two contributions so as to enhance the overall sensitivity.
Therefore, The comparative low sensitivity in the proposed TUMRR is mainly due to complex design of the configuration which results in dropping of waveguide sensitivity and hence overall sensitivity is reduced. Also, it has been reported that cascaded micro-ring resonators exhibit a wide transmission band rather than sharp resonances, and, thus, do not allow the quality factor to be improved without exploiting any other physical effect (i.e. a Fano resonance). (Ciminelli et al. 2013).
Figure of merit can be expressed as (Al Mahmod et al. 2018);
| 14 |
The mathematical expression for calculating Limit of Detection (LOD) is (Al Mahmod et al. 2018);
| 15 |
where factor “f” symbolizes the fraction of resonance line-width “δλ” that can be resolved by the detection system which taken as f = 1/400 (Al Mahmod et al. 2018).
Extinction ratio (ER) can be expressed as (Al Mahmod et al. 2018; Ranjan et al. 2021);
| 16 |
where PL and PH are the lowest amplitude and highest amplitude of the resonant peak. The computed value of ER = 3.01 dB.
The field analysis for Off-Resonance and On-Resonance condition is shown in Figs. 7 and 8 respectively.
Fig. 7.

Field analysis for Off-Resonance condition TUMRR
Fig. 8.

Field analysis for On-Resonance condition TUMRR
3-D shematics of proposed TUMRR representing lateral and vertical coupling is shown in Fig. 9
Fig. 9.

3D schematics representing lateral and vertical coupling of TUMRR
Conclusion
The offered triple unsymmetrical micro ring resonators are designed in z-domain and its transfer function is realized using Mason’s gain rule. The performance of the offered TUMRR is determined using MATLAB software. The FSR is evaluated from the frequency response investigation using MATLAB environment for single input and single output arrangement, i.e., Tf is 243.5 GHz. The proposed TUMRR provides a proportional shift in resonant wavelength when analytes of different refractive index is placed at resonator surface. The sensitivity of the proposed TUMRR based biosensor is around 200–280 nm/RIU for different blood cells where as the Q-factor attained is 1938. The proposed model can help to improve data handling capacity of an optical communication system as well as the presented results are promising candidate for biosensing applications.
Appendix
Single Loop
Two non-touching Loop
Three Non-touching loop
Forward Path and Path Factor
Author contributions
SK: Conceptualization, Methodology, Software, Validation. KS: Data curation,Investigation, Formal analysis. SR: Supervision, Writing—review & editing, Project administration.
Funding
No direct or indirect funding is received for this article.
Data availability
Not applicable.
Declarations
Conflict of interest
On behalf of all authors, the corresponding author states that there is no financial or personal conflict of interest.
Footnotes
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
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