Antibodies |
|
Goat anti-human IgG, 1:4000 |
Jackson |
109-035-098; RRID: AB_2337586
|
Goat anti-human IgA, 1:750 |
Thermo Fisher Scientific |
31417; RRID: AB_228253
|
|
Chemicals, peptides, and recombinant proteins |
|
WT SARS-CoV-2 Spike ECD |
Oxford, SGC |
N/A |
WT SARS-CoV-2 RBD |
Oxford, SGC |
N/A |
WT SARS-CoV-2 NC |
AcroBiosystems |
NUN-C5227 |
WT SARS-CoV-2 Spike prefusion ECD |
Lausanne, EPFL SV PTECH PTPSP and Zurich, UZH |
N/A |
WT SARS-CoV-2 RBD |
Trenzyme |
P2020-001 |
WT SARS-CoV-2 NSP1 |
Zurich, ETH |
N/A |
CMV pp65 |
Abcam |
ab43041 |
Bovine serum albumin (BSA) |
Thermo Fisher Scientific |
23209 |
SARS-CoV (2003) RBD |
Yale, New Haven |
N/A |
|
Critical commercial assays |
|
SARS-CoV-2 nucleocapsid test on E801 of the COBAS8000® system |
Roche diagnostics |
N/A |
LIAISON® SARS-CoV-2 chemiluminescence immunoassay |
DiaSorin |
N/A |
SARS-CoV-2 chemiluminescent microparticle immunoassay on an Architect™ analyser |
Abbott |
N/A |
IgA or IgG against the SARS-CoV-2 S1 antigen on DSX™ Automated ELISA System |
EUROIMMUN and DYNEX |
N/A |
High-throughput serology assay in Oxford (under development) |
Final version of assay published by The National SARS-CoV-2 Serology Assay Evaluation Group80 and described here.81
|
N/A |
|
Experimental models: Cell lines |
|
Expi239F cells |
Thermo Fisher |
A14527 |
ExpiCHO cells |
Thermo Fisher |
A29127 |
Vero E6 cells |
ATCC |
CRL-1586 |
|
Software and algorithms |
|
Python 3 |
Python Software Foundation |
N/A |
R 4.2.0 statistical software |
R Core Team |
N/A |
R Studio 2022.07.1 Build 554 |
R Studio, PBC |
N/A |
Stan82
|
Stan development team |
N/A |
Code used in the current study |
Zenodo repository1
|
https://doi.org/10.5281/zenodo.7454292 |
GraphPad |
Prism |
N/A |
|
Other |
|
1536-well high-binding ELISA plates (SpectraPlate) |
Perkin Elmer |
6004500 |
384-well high-binding ELISA plates (SpectraPlate) |
Perkin Elmer |
6007500 |
384-well low-binding PP plates |
Labcyte/Beckman Coulter |
001-14555 |
ECHO 555 Acoustic Dispenser |
Labcyte/Beckman Coulter |
N/A |
El406 Washer/Dispenser |
Biotek |
N/A |
MultifloFX |
Biotek |
N/A |
Certus Flex dispenser |
Fritz Gyger AG |
N/A |
Microplate Centrifuge |
Agilent |
G5582AA |
PlateLoc thermal microplate sealer |
Agilent |
G5585BA |
XPeel automated plate seal removal |
Brooks/Azenta |
N/A |
EnVision multimode plate reader |
Perkin Elmer |
2105-0010 |
Fluidity One-W |
Fluidic Analytics |
N/A |
iBlot 2 Gel Transfer Device |
Thermo Fisher |
IB21001 |
Fusion SOLO S imaging system |
Vilber |
N/A |