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. 2023 Jan 4;26(2):105928. doi: 10.1016/j.isci.2023.105928
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Goat anti-human IgG, 1:4000 Jackson 109-035-098; RRID: AB_2337586
Goat anti-human IgA, 1:750 Thermo Fisher Scientific 31417; RRID: AB_228253

Chemicals, peptides, and recombinant proteins

WT SARS-CoV-2 Spike ECD Oxford, SGC N/A
WT SARS-CoV-2 RBD Oxford, SGC N/A
WT SARS-CoV-2 NC AcroBiosystems NUN-C5227
WT SARS-CoV-2 Spike prefusion ECD Lausanne, EPFL SV PTECH PTPSP and Zurich, UZH N/A
WT SARS-CoV-2 RBD Trenzyme P2020-001
WT SARS-CoV-2 NSP1 Zurich, ETH N/A
CMV pp65 Abcam ab43041
Bovine serum albumin (BSA) Thermo Fisher Scientific 23209
SARS-CoV (2003) RBD Yale, New Haven N/A

Critical commercial assays

SARS-CoV-2 nucleocapsid test on E801 of the COBAS8000® system Roche diagnostics N/A
LIAISON® SARS-CoV-2 chemiluminescence immunoassay DiaSorin N/A
SARS-CoV-2 chemiluminescent microparticle immunoassay on an Architect™ analyser Abbott N/A
IgA or IgG against the SARS-CoV-2 S1 antigen on DSX™ Automated ELISA System EUROIMMUN and DYNEX N/A
High-throughput serology assay in Oxford (under development) Final version of assay published by The National SARS-CoV-2 Serology Assay Evaluation Group80 and described here.81 N/A

Experimental models: Cell lines

Expi239F cells Thermo Fisher A14527
ExpiCHO cells Thermo Fisher A29127
Vero E6 cells ATCC CRL-1586

Software and algorithms

Python 3 Python Software Foundation N/A
R 4.2.0 statistical software R Core Team N/A
R Studio 2022.07.1 Build 554 R Studio, PBC N/A
Stan82 Stan development team N/A
Code used in the current study Zenodo repository1 https://doi.org/10.5281/zenodo.7454292
GraphPad Prism N/A

Other

1536-well high-binding ELISA plates (SpectraPlate) Perkin Elmer 6004500
384-well high-binding ELISA plates (SpectraPlate) Perkin Elmer 6007500
384-well low-binding PP plates Labcyte/Beckman Coulter 001-14555
ECHO 555 Acoustic Dispenser Labcyte/Beckman Coulter N/A
El406 Washer/Dispenser Biotek N/A
MultifloFX Biotek N/A
Certus Flex dispenser Fritz Gyger AG N/A
Microplate Centrifuge Agilent G5582AA
PlateLoc thermal microplate sealer Agilent G5585BA
XPeel automated plate seal removal Brooks/Azenta N/A
EnVision multimode plate reader Perkin Elmer 2105-0010
Fluidity One-W Fluidic Analytics N/A
iBlot 2 Gel Transfer Device Thermo Fisher IB21001
Fusion SOLO S imaging system Vilber N/A